Methods of SARS-CoV-2 Inactivation.

Anderson, Enyia R; Prince, Tessa; Turtle, Lance; Hughes, Grant L; Patterson, Edward I

Anderson, Enyia R; Prince, Tessa; Turtle, Lance; Hughes, Grant L; Patterson, Edward I.

Anderson ER; Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
Prince T; Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
Turtle L; Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Liverpool, UK.
Hughes GL; NIHR Health Protection Unit in Emerging and Zoonotic Infections, Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, UK.
Patterson EI; NIHR Health Protection Unit in Emerging and Zoonotic Infections, Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, UK.

Methods Mol Biol ; 2452: 465-473, 2022.

Article in English | MEDLINE | ID: covidwho-1844280

ABSTRACT

ABSTRACT

Inactivation methods allow for hazard group 3 (HG3) pathogens to be disposed of and used safely in downstream experiments and assays to be carried out at lower containment levels. Commonly used viral inactivation methods include heat inactivation, fixation methods, ultraviolet (UV) light and detergent inactivation. Here we describe known methods used to inactivate SARS-CoV-2 for safe downstream biological assays.

Subject(s)

COVID-19; SARS-CoV-2; Animals; Chlorocebus aethiops; Ultraviolet Rays; Vero Cells; Virus Inactivation

Keywords

Assay; Inactivation; SARS-CoV-2; TRIzol; UV; Vero

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Animals Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: 978-1-0716-2111-0_25

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