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Bacteriological and molecular detectionof Compylobacter species in human and animals in Diyala province
Biochemical and Cellular Archives ; 22(1):1075-1092, 2022.
Article in English | CAB Abstracts | ID: covidwho-1848276
ABSTRACT
Campylobacteriosis is one of the most well-characterized bacterial foodborne infections worldwide, the species are the most common zoonotic pathogens. Chicken and chicken products are known to be the major sources of Campylobacter infection in humans. Campylobacter species represent one of the most common causes of bacterial diarrheal illness worldwide, it is the main cause of bacterial gastrointestinal infections occurring at any age, but it peaks in children and young adults. This study aims to investigate the presence and prevalence of Campylobacter bacteria in humans and chicken and aims for determination the extent of the contamination of this bacteria. The study involved two parts;the first part designed for isolation Campylobacter species from chickens which were alive, while the second part was about isolation Campylobacter species from human stool samples for different ages. The current study based on analysis of three hundred and fifty (350) stool samples taken from humans with different ages. The patients samples were collected from Al-Batool Teaching Hospital for Maternity and Pediatric, Baquba Teaching Hospital and Central Health Laboratories in Diyala, these patients were had vary clinical aspects like (diarrhea, Fever, nausea, vomiting, abdominal pain and cramp, fatigue and muscle pain). On the other hand, one hundred seventy five (175) stool samples of chicken which they apparently had diarrhea were collected randomly from different areas of Diyala Governorate. The collection of both humans and chicken samples were during 2020 - 2021. The analysis of 350 samples of patients their ages ranged from 3 months to 59 years showed that the mean +or- SD of age was 24.5 +or- 14.3 years. The most predominant age group was 20-29 years (26.9%), while the lowest age group was 50-59 years (4.9%). Regarding the gender, 151 (43.1%) of the included patients were male and 199 (56.9%) were female. Patients included in the current study were collected from four districts of Diyala province. The highest collection rate was from Baquba district 217(62.0%), while the lowest collection rate was from Baladrooz district 18 (5.1%). the distribution of clinical signs and symptoms of included patients. Persistent diarrhea forming the predominance of signs 179 (51.1%), followed by watery diarrhea 55 (15.7%), while the least sign was vomiting 1(3%). Furthermore, 15 (4.3%) of patients showed all that signs and symptoms. The signs and symptoms were diarrhea was found in 194 (55.4%) patients, followed by watery diarrhea in 77 (22.0%) patients. Fever was found in 30 (8.6%) patients. The least sign was nausea which was found in 15 (4.3%) patients. Abdominal pain and cramps were detected in 22 (6.3%) patients. Out of 350 stool specimens submitted for bacteriological culture, 62 (17.7%) were positive versus 288 (82.3%) were negative. Among the 62-culture positive, 13 specimens yield Campylobacter growth, so the campylobacter detection rate among human specimens was 3.7%. The isolated Campylobacter were as follow;11(3.1%) were Campylobacter jejuni, one (0.3%) was Campylobacter coli, one (0.3%) was undifferentiated campylobacter. On the other hand, 58 (16.6%) of the specimens yield bacterial growth other than Campylobacter, as follows E. coli was detected in 28 (8.0%), Proteus Spp. was detected in 12 (3.4%), H. pylori was detected in 10 (2.9%) and Klebsiella Spp. was detected in 8 (2.3%). The remaining specimens 279 (79.7%) yield no growth. The results of the PCR technique revealed that 8 (2.38%) specimens were positive for Campylobacter, while the majority 335 (95.7%) were negative and 7(2.0%) of the specimens were not done. While the analysis of 175 chicken fecal samples taken from chickens were mainly suffering from diarrhea showed that the distribution of chickens according to their ages in days showed predominance was those 30-39 (40.6%) days old. It is clear that the majority of specimens (74.3%) were collected from Baquba districts. The results showed that specimens collected Al- Muqdadiya district farms had yielded the high
Keywords
Prion, Viral, Bacterial and Fungal Pathogens of Humans [VV210]; Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821]; Food Contamination, Residues and Toxicology [QQ200]; human diseases; disease prevalence; epidemiology; bacterial diseases; children; patients; infections; clinical aspects; symptoms; risk factors; viral diseases; abdomen; campylobacteriosis; detection; diarrhoea; digestive system; distribution; foodborne diseases; gastrointestinal diseases; hospitals; human faeces; muscles; nausea; pain; pathogens; vomiting; zoonoses; microbial contamination; age groups; sex; poultry; man; Severe acute respiratory syndrome coronavirus 2; Campylobacter jejuni; animals; Campylobacter coli; Campylobacter lari; fowls; Klebsiella; Proteus (Bacteria); Escherichia coli; Iraq; Homo; Hominidae; primates; mammals; vertebrates; Chordata; eukaryotes; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Campylobacter; Campylobacteraceae; Campylobacterales; Epsilonproteobacteria; Proteobacteria; Bacteria; prokaryotes; Gallus gallus; Gallus; Phasianidae; Galliformes; birds; Enterobacteriaceae; Enterobacteriales; Gammaproteobacteria; medium Human Development Index countries; Middle East; upper-middle income countries; West Asia; Asia; Escherichia; bacterial infections; bacterioses; bacterium; clinical picture; SARS-CoV-2; viral infections; diarrhea; scouring; alimentary tract; gastrointestinal system; chickens; human feces; zoonotic infections; E. coli; domesticated birds
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Collection: Databases of international organizations Database: CAB Abstracts Language: English Journal: Biochemical and Cellular Archives Year: 2022 Document Type: Article

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Collection: Databases of international organizations Database: CAB Abstracts Language: English Journal: Biochemical and Cellular Archives Year: 2022 Document Type: Article