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Application of digital PCR to determine the reliability of Xpert Xpress SARS-CoV-2 assay with envelope (E) gene negative and nucleocapsid (N2) gene positive results.
Wong, River Chun-Wai; Wong, Ann Han; Ho, Yolanda Iok-Ieng; Siu, Gilman Kit-Hang; Lee, Lam-Kwong; Leung, Eddie Chi-Man; Lai, Raymond Wai-Man.
  • Wong RC; Department of Microbiology, Prince of Wales Hospital, Hong Kong. Electronic address: wcw372@ha.org.hk.
  • Wong AH; Department of Microbiology, Prince of Wales Hospital, Hong Kong.
  • Ho YI; Department of Microbiology, Prince of Wales Hospital, Hong Kong.
  • Siu GK; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong.
  • Lee LK; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong.
  • Leung EC; Department of Microbiology, Prince of Wales Hospital, Hong Kong.
  • Lai RW; Department of Microbiology, Prince of Wales Hospital, Hong Kong.
Diagn Microbiol Infect Dis ; 103(4): 115726, 2022 Aug.
Article in English | MEDLINE | ID: covidwho-1889344
ABSTRACT
This study used digital polymerase chain reaction (dPCR) to determine whether envelope (E) gene-negative and nucleocapsid (N2) gene-positive (E-N+) results obtained with the Cepheid Xpert Xpress SARS-CoV-2 assay are reliable. Using droplet digital PCR results as a reference, 18 of 22 E-N+ samples with a low viral load (81.8%) were identified as true positives.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article