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Longitudinal SARS-CoV-2 RNA wastewater monitoring across a range of scales correlates with total and regional COVID-19 burden in a well-defined urban population.
Acosta, Nicole; Bautista, María A; Waddell, Barbara J; McCalder, Janine; Beaudet, Alexander Buchner; Man, Lawrence; Pradhan, Puja; Sedaghat, Navid; Papparis, Chloe; Bacanu, Andra; Hollman, Jordan; Krusina, Alexander; Southern, Danielle A; Williamson, Tyler; Li, Carmen; Bhatnagar, Srijak; Murphy, Sean; Chen, Jianwei; Kuzma, Darina; Clark, Rhonda; Meddings, Jon; Hu, Jia; Cabaj, Jason L; Conly, John M; Dai, Xiaotian; Lu, Xuewen; Chekouo, Thierry; Ruecker, Norma J; Achari, Gopal; Ryan, M Cathryn; Frankowski, Kevin; Hubert, Casey R J; Parkins, Michael D.
  • Acosta N; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada.
  • Bautista MA; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Waddell BJ; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada.
  • McCalder J; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Beaudet AB; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Man L; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Pradhan P; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Sedaghat N; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Papparis C; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Bacanu A; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Hollman J; Department of Civil Engineering, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada; Department of Geosciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Krusina A; Department of Medicine, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada.
  • Southern DA; Department of Medicine, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada.
  • Williamson T; Department of Community Health Sciences, University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta, T2N 4Z6, Canada; O'Brien Institute for Public Health, University of Calgary, Calgary, 3280 Hospital Dr NW, Calgary, Alberta, T2N 4Z6, Canada.
  • Li C; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Bhatnagar S; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Murphy S; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Chen J; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Kuzma D; Advancing Canadian Water Assets, University of Calgary, 3131 210 Ave SE, Calgary, Alberta, T0L 0×0, Canada.
  • Clark R; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada; Advancing Canadian Water Assets, University of Calgary, 3131 210 Ave SE, Calgary, Alberta, T0L 0×0, Canada.
  • Meddings J; Department of Medicine, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada.
  • Hu J; Department of Community Health Sciences, University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta, T2N 4Z6, Canada; O'Brien Institute for Public Health, University of Calgary, Calgary, 3280 Hospital Dr NW, Calgary, Alberta, T2N 4Z6, Canada; Provincial Population & Public Health, Alberta H
  • Cabaj JL; Department of Community Health Sciences, University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta, T2N 4Z6, Canada; O'Brien Institute for Public Health, University of Calgary, Calgary, 3280 Hospital Dr NW, Calgary, Alberta, T2N 4Z6, Canada; Provincial Population & Public Health, Alberta H
  • Conly JM; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Department of Medicine, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada; O'Brien Institute for Pub
  • Dai X; Department of Mathematics and Statistics, University of Calgary, 2500 Hospital Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Lu X; Department of Mathematics and Statistics, University of Calgary, 2500 Hospital Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Chekouo T; Department of Mathematics and Statistics, University of Calgary, 2500 Hospital Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Ruecker NJ; Water Quality Services, City of Calgary, 625 25 Ave SE, Calgary, Alberta, T2G 4k8, Canada.
  • Achari G; Department of Civil Engineering, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Ryan MC; Department of Geosciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Frankowski K; Advancing Canadian Water Assets, University of Calgary, 3131 210 Ave SE, Calgary, Alberta, T0L 0×0, Canada.
  • Hubert CRJ; Geomicrobiology Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada.
  • Parkins MD; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada; Department of Medicine, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada; Snyder Institute for Chro
Water Res ; 220: 118611, 2022 Jul 15.
Article in English | MEDLINE | ID: covidwho-1852230
ABSTRACT
Wastewater-based epidemiology (WBE) is an emerging surveillance tool that has been used to monitor the ongoing COVID-19 pandemic by tracking SARS-CoV-2 RNA shed into wastewater. WBE was performed to monitor the occurrence and spread of SARS-CoV-2 from three wastewater treatment plants (WWTP) and six neighborhoods in the city of Calgary, Canada (population 1.44 million). A total of 222 WWTP and 192 neighborhood samples were collected from June 2020 to May 2021, encompassing the end of the first-wave (June 2020), the second-wave (November end to December 2020) and the third-wave of the COVID-19 pandemic (mid-April to May 2021). Flow-weighted 24-hour composite samples were processed to extract RNA that was then analyzed for two SARS-CoV-2-specific regions of the nucleocapsid gene, N1 and N2, using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Using this approach SARS-CoV-2 RNA was detected in 98.06% (406/414) of wastewater samples. SARS-CoV-2 RNA abundance was compared to clinically diagnosed COVID-19 cases organized by the three-digit postal code of affected individuals' primary residences, enabling correlation analysis at neighborhood, WWTP and city-wide scales. Strong correlations were observed between N1 & N2 gene signals in wastewater and new daily cases for WWTPs and neighborhoods. Similarly, when flow rates at Calgary's three WWTPs were used to normalize observed concentrations of SARS-CoV-2 RNA and combine them into a city-wide signal, this was strongly correlated with regionally diagnosed COVID-19 cases and clinical test percent positivity rate. Linked census data demonstrated disproportionate SARS-CoV-2 in wastewater from areas of the city with lower socioeconomic status and more racialized communities. WBE across a range of urban scales was demonstrated to be an effective mechanism of COVID-19 surveillance.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Water Res Year: 2022 Document Type: Article Affiliation country: J.watres.2022.118611

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Water Res Year: 2022 Document Type: Article Affiliation country: J.watres.2022.118611