Your browser doesn't support javascript.
SARS-CoV-2 infection results in immune responses in the respiratory tract and peripheral blood that suggest mechanisms of disease severity.
Zhang, Wuji; Chua, Brendon Y; Selva, Kevin J; Kedzierski, Lukasz; Ashhurst, Thomas M; Haycroft, Ebene R; Shoffner-Beck, Suzanne K; Hensen, Luca; Boyd, David F; James, Fiona; Mouhtouris, Effie; Kwong, Jason C; Chua, Kyra Y L; Drewett, George; Copaescu, Ana; Dobson, Julie E; Rowntree, Louise C; Habel, Jennifer R; Allen, Lilith F; Koay, Hui-Fern; Neil, Jessica A; Gartner, Matthew J; Lee, Christina Y; Andersson, Patiyan; Khan, Sadid F; Blakeway, Luke; Wisniewski, Jessica; McMahon, James H; Vine, Erica E; Cunningham, Anthony L; Audsley, Jennifer; Thevarajan, Irani; Seemann, Torsten; Sherry, Norelle L; Amanat, Fatima; Krammer, Florian; Londrigan, Sarah L; Wakim, Linda M; King, Nicholas J C; Godfrey, Dale I; Mackay, Laura K; Thomas, Paul G; Nicholson, Suellen; Arnold, Kelly B; Chung, Amy W; Holmes, Natasha E; Smibert, Olivia C; Trubiano, Jason A; Gordon, Claire L; Nguyen, Thi H O.
  • Zhang W; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Chua BY; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Selva KJ; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan.
  • Kedzierski L; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Ashhurst TM; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Haycroft ER; Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Victoria, 3000, Australia.
  • Shoffner-Beck SK; Sydney Cytometry Core Research Facility, Charles Perkins Centre, Centenary Institute and University of Sydney, Sydney, NSW, Australia.
  • Hensen L; Sydney Institute for Infectious Diseases, University of Sydney, Sydney, NSW, Australia.
  • Boyd DF; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • James F; Department of Biomedical Engineering, University of Michigan, Michigan, USA.
  • Mouhtouris E; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Kwong JC; Department of Immunology, St Jude Children's Research Hospital, Memphis, TN, USA.
  • Chua KYL; Department of Infectious Diseases, Austin Health, Heidelberg, VIC, Australia.
  • Drewett G; Department of Infectious Diseases, Austin Health, Heidelberg, VIC, Australia.
  • Copaescu A; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Dobson JE; Department of Infectious Diseases, Austin Health, Heidelberg, VIC, Australia.
  • Rowntree LC; Department of Infectious Diseases, Austin Health, Heidelberg, VIC, Australia.
  • Habel JR; Department of Infectious Diseases, Austin Health, Heidelberg, VIC, Australia.
  • Allen LF; Department of Infectious Diseases, Austin Health, Heidelberg, VIC, Australia.
  • Koay HF; Department of Radiology, Austin Health, Heidelberg, VIC, Australia.
  • Neil JA; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Gartner MJ; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Lee CY; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Andersson P; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Khan SF; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Blakeway L; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Wisniewski J; Department of Biomedical Engineering, University of Michigan, Michigan, USA.
  • McMahon JH; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
  • Vine EE; Department of Infectious Diseases, Monash University and Alfred Hospital, Melbourne, VIC, Australia.
  • Cunningham AL; Department of Infectious Diseases, Monash University and Alfred Hospital, Melbourne, VIC, Australia.
  • Audsley J; Department of Infectious Diseases, Monash University and Alfred Hospital, Melbourne, VIC, Australia.
  • Thevarajan I; Department of Infectious Diseases, Monash University and Alfred Hospital, Melbourne, VIC, Australia.
  • Seemann T; Department of Infectious Diseases, Monash Medical Centre, Melbourne, VIC, Australia.
  • Sherry NL; Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, NSW, Australia.
  • Amanat F; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia.
  • Krammer F; Sydney Infectious Diseases, Faculty of Medicine and Health, University of Sydney, Westmead, NSW, Australia.
  • Londrigan SL; Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, NSW, Australia.
  • Wakim LM; Sydney Infectious Diseases, Faculty of Medicine and Health, University of Sydney, Westmead, NSW, Australia.
  • King NJC; Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, 3000, Australia.
  • Godfrey DI; Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, 3000, Australia.
  • Mackay LK; Victorian Infectious Diseases Services, The Royal Melbourne Hospital and Doherty Department University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, 3000, VIC, Australia.
  • Thomas PG; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
  • Nicholson S; Department of Infectious Diseases, Austin Health, Heidelberg, VIC, Australia.
  • Arnold KB; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
  • Chung AW; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Holmes NE; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Smibert OC; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Trubiano JA; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Gordon CL; Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
  • Nguyen THO; Sydney Cytometry Core Research Facility, Charles Perkins Centre, Centenary Institute and University of Sydney, Sydney, NSW, Australia.
Nat Commun ; 13(1): 2774, 2022 05 19.
Article in English | MEDLINE | ID: covidwho-1900484
ABSTRACT
Respiratory tract infection with SARS-CoV-2 results in varying immunopathology underlying COVID-19. We examine cellular, humoral and cytokine responses covering 382 immune components in longitudinal blood and respiratory samples from hospitalized COVID-19 patients. SARS-CoV-2-specific IgM, IgG, IgA are detected in respiratory tract and blood, however, receptor-binding domain (RBD)-specific IgM and IgG seroconversion is enhanced in respiratory specimens. SARS-CoV-2 neutralization activity in respiratory samples correlates with RBD-specific IgM and IgG levels. Cytokines/chemokines vary between respiratory samples and plasma, indicating that inflammation should be assessed in respiratory specimens to understand immunopathology. IFN-α2 and IL-12p70 in endotracheal aspirate and neutralization in sputum negatively correlate with duration of hospital stay. Diverse immune subsets are detected in respiratory samples, dominated by neutrophils. Importantly, dexamethasone treatment does not affect humoral responses in blood of COVID-19 patients. Our study unveils differential immune responses between respiratory samples and blood, and shows how drug therapy affects immune responses during COVID-19.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2022 Document Type: Article Affiliation country: S41467-022-30088-y

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2022 Document Type: Article Affiliation country: S41467-022-30088-y