MULTISCALE FRACTAL SHAPE CUES SUPPORT HIERARCHICAL ASSEMBLY AND MATURATION OF PODOCYTES VIA CURVATURE-INDUCED EXTRACELLULAR MATRIX PATTERNING
Tissue Engineering - Part A
; 28(SUPPL 1):S648, 2022.
Article
in English
| EMBASE | ID: covidwho-1852883
ABSTRACT
Nature efficiently self-organizes cells and tissues into complex fractal forms. Whether fractal patterning contributes functionally to maturation, and how cells sense and interpret such shape cues, is not well understood. Using kidney podocytes as a model system, bioinspired templating of glomerular histology was leveraged to design controlled fractal 21/2 -D surfaces for cell culture. Microcurvature was associated with charge density gradients in space, found to direct extracellular matrix protein organization resulting in hierarchical assembly of cell structures and fractally-branching podocyte morphology in vitro, outlined with a novel fluorescent assaying technique. Shape simulation was uniquely associated with mature-like foot processes on an organized ECM. In applications of drug testing, coronavirus infection, and a cells-as-sensors approach to patient serum diagnostics, fractally stimulated cells were more responsive than flat cultures. Fractal frameworks may thus provide a functional role in podocyte maturation and could serve to advance other bioengineered systems.
endogenous compound; scleroprotein; adult; conference abstract; controlled study; Coronavirus infection; density gradient; drug screening; extracellular matrix; foot; fractal analysis; histology; histopathology; human; human cell; human tissue; in vitro study; kidney cell culture; podocyte; sensor; simulation
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Tissue Engineering - Part A
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS