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Remote Floating-Gate Field-Effect Transistor with 2-Dimensional Reduced Graphene Oxide Sensing Layer for Reliable Detection of SARS-CoV-2 Spike Proteins.
Jang, Hyun-June; Sui, Xiaoyu; Zhuang, Wen; Huang, Xiaodan; Chen, Min; Cai, Xiaolei; Wang, Yale; Ryu, Byunghoon; Pu, Haihui; Ankenbruck, Nicholas; Beavis, Kathleen; Huang, Jun; Chen, Junhong.
  • Jang HJ; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
  • Sui X; Chemical Sciences and Engineering Division, Physical Sciences and Engineering Directorate, Argonne National Laboratory, Lemont, Illinois 60439, United States.
  • Zhuang W; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
  • Huang X; Chemical Sciences and Engineering Division, Physical Sciences and Engineering Directorate, Argonne National Laboratory, Lemont, Illinois 60439, United States.
  • Chen M; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
  • Cai X; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
  • Wang Y; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
  • Ryu B; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
  • Pu H; Department of Mechanical Engineering, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, United States.
  • Ankenbruck N; Chemical Sciences and Engineering Division, Physical Sciences and Engineering Directorate, Argonne National Laboratory, Lemont, Illinois 60439, United States.
  • Beavis K; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
  • Huang J; Chemical Sciences and Engineering Division, Physical Sciences and Engineering Directorate, Argonne National Laboratory, Lemont, Illinois 60439, United States.
  • Chen J; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, United States.
ACS Appl Mater Interfaces ; 14(21): 24187-24196, 2022 Jun 01.
Article in English | MEDLINE | ID: covidwho-1860277
ABSTRACT
Despite intensive research of nanomaterials-based field-effect transistors (FETs) as a rapid diagnostic tool, it remains to be seen for FET sensors to be used for clinical applications due to a lack of stability, reliability, reproducibility, and scalability for mass production. Herein, we propose a remote floating-gate (RFG) FET configuration to eliminate device-to-device variations of two-dimensional reduced graphene oxide (rGO) sensing surfaces and most of the instability at the solution interface. Also, critical mechanistic factors behind the electrochemical instability of rGO such as severe drift and hysteresis were identified through extensive studies on rGO-solution interfaces varied by rGO thickness, coverage, and reduction temperature. rGO surfaces in our RFGFET structure displayed a Nernstian response of 54 mV/pH (from pH 2 to 11) with a 90% yield (9 samples out of total 10), coefficient of variation (CV) < 3%, and a low drift rate of 2%, all of which were calculated from the absolute measurement values. As proof-of-concept, we demonstrated highly reliable, reproducible, and label-free detection of spike proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a saliva-relevant media with concentrations ranging from 500 fg/mL to 5 µg/mL, with an R2 value of 0.984 and CV < 3%, and a guaranteed limit of detection at a few pg/mL. Taken together, this new platform may have an immense effect on positioning FET bioelectronics in a clinical setting for detecting SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article Affiliation country: Acsami.2c04969

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article Affiliation country: Acsami.2c04969