AntiV-SGN: a universal antiviral strategy to combat both RNA and DNA viruses by destroying their nucleic acids without sequence limitation.
Microb Biotechnol
; 15(9): 2488-2501, 2022 09.
Article
in English
| MEDLINE | ID: covidwho-1864192
ABSTRACT
Numerous viral outbreaks have threatened us throughout history. Here, we demonstrated a nucleic acid-based antiviral strategy named AntiV-SGN. Unlike those CRISPR-mediated methods, AntiV-SGN has advantages of no targets' sequence limitation, such as protospacer adjacent motif (PAM) or protospacer flanking sequence (PFS), being universal for both DNA and RNA viruses. AntiV-SGN was composed of a FEN1 protein and specific hpDNAs targeting viruses' nucleic acid. Its antiviral ability was tested on SARS-CoV-2 and HBV respectively. Reporter assays in human cells first illustrated the feasibility of AntiV-SGN. Then, it was verified that AntiV-SGN destroyed about 50% of live RNAs of SARS-CoV-2 in Vero cells and 90% cccDNA of HBV in HepG2.2.15 cells. It was also able to remove viral DNA integrated into the host's genome. In the mouse model, AntiV-SGN can be used to significantly reduce HBV expression at a level of 90%. Actually, in some cases, when viruses mutate to eliminate PAM/PFS or hosts were infected by both DNA and RNA viruses, AntiV-SGN could be a choice. Collectively, this study provided a proof-of-concept antiviral strategy of AntiV-SGN, which has potential clinical value for targeting a wide variety of human pathogens, both known and newly identified.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viruses
/
Nucleic Acids
/
COVID-19
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Microb Biotechnol
Year:
2022
Document Type:
Article
Affiliation country:
1751-7915.14076
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