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Circularized fluorescent nanodiscs for probing protein-lipid interactions.
Ren, Qian; Zhang, Shanwen; Bao, Huan.
  • Ren Q; Department of Molecular Medicine, UF Scripps Biomedical Research, 130 Scripps Way, Jupiter, 33458, FL, USA.
  • Zhang S; Department of Molecular Medicine, UF Scripps Biomedical Research, 130 Scripps Way, Jupiter, 33458, FL, USA.
  • Bao H; Department of Molecular Medicine, UF Scripps Biomedical Research, 130 Scripps Way, Jupiter, 33458, FL, USA. baoh@ufl.edu.
Commun Biol ; 5(1): 507, 2022 05 26.
Article in English | MEDLINE | ID: covidwho-1864775
ABSTRACT
Protein-lipid interactions are vital for numerous transmembrane signaling pathways. However, simple tools to characterize these interactions remain scarce and are much needed to advance our understanding of signal transduction across lipid bilayers. To tackle this challenge, we herein engineer nanodisc as a robust fluorescent sensor for reporting membrane biochemical reactions. We circularize nanodiscs via split GFP and thereby create an intensity-based fluorescent sensor (isenND) for detecting membrane binding and remodeling events. We show that isenND responds robustly and specifically to the action of a diverse array of membrane-interacting proteins and peptides, ranging from synaptotagmin and synuclein involved in neurotransmission to viral fusion peptides of HIV-1 and SARS-CoV-2. Together, isenND can serve as a versatile biochemical reagent useful for basic and translational research of membrane biology.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanostructures / COVID-19 Limits: Humans Language: English Journal: Commun Biol Year: 2022 Document Type: Article Affiliation country: S42003-022-03443-4

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanostructures / COVID-19 Limits: Humans Language: English Journal: Commun Biol Year: 2022 Document Type: Article Affiliation country: S42003-022-03443-4