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Quantitative and ultrasensitive in situ immunoassay technology for SARS-CoV-2 detection in saliva.
Chen, Yuchao; Liu, Fei; Lee, Luke P.
  • Chen Y; WellSIM Biomedical Technologies Inc., 626 Bancroft Way, Suite A, Berkeley, CA, USA.
  • Liu F; WellSIM Biomedical Technologies Inc., 626 Bancroft Way, Suite A, Berkeley, CA, USA.
  • Lee LP; Harvard Medical School, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
Sci Adv ; 8(21): eabn3481, 2022 05 27.
Article in English | MEDLINE | ID: covidwho-1865136
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic has become an immense global health crisis. However, the lack of efficient and sensitive on-site testing methods limits early detection for timely isolation and intervention. Here, we present a quantitative and ultrasensitive in situ immunoassay technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in saliva (QUIT SARS-CoV-2). Our nanoporous membrane resonator generates a rapid oscillating flow to purify and concentrate fully intact SARS-CoV-2 virus in saliva by 40-fold for in situ detection of viral antigens based on chemiluminescent immunoassay within 20 min. This method can not only achieve a detection sensitivity below 100 copies/ml of virus, comparable to the bench-top PCR equipment; it can also improve detection specificity via direct monitoring of viral loads. The integrated portable QUIT SARS-CoV-2 system, which enables rapid and accurate on-site viral screening with a high-throughput sample pooling strategy, can be performed in primary care settings and substantially improve the detection and prevention of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Adv Year: 2022 Document Type: Article Affiliation country: Sciadv.abn3481

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Adv Year: 2022 Document Type: Article Affiliation country: Sciadv.abn3481