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Isolation of SARS-CoV-2 from COVID-19 Patients and an Asymptomatic Individual.
Hishiki, Takayuki; Usui, Kengo; An, Tadaichi; Suzuki, Rieko; Sakuragi, Jun-Ichi; Tanaka, Yuki; Matsuki, Yu; Kawai, Jun; Kogo, Yasushi; Hayashizaki, Yoshihide; Takasaki, Tomohiko.
  • Hishiki T; Department of Microbiology, Kanagawa Prefectural Institute of Public Health, Japan.
  • Usui K; RIKEN Center for Integrative Medical Science, Japan.
  • An T; RIKEN Center for Integrative Medical Science, Japan.
  • Suzuki R; Department of Microbiology, Kanagawa Prefectural Institute of Public Health, Japan.
  • Sakuragi JI; Department of Microbiology, Kanagawa Prefectural Institute of Public Health, Japan.
  • Tanaka Y; RIKEN Center for Integrative Medical Science, Japan.
  • Matsuki Y; RIKEN Center for Integrative Medical Science, Japan.
  • Kawai J; RIKEN Preventive Medicine and Diagnosis Innovation Program, Japan.
  • Kogo Y; RIKEN Preventive Medicine and Diagnosis Innovation Program, Japan.
  • Hayashizaki Y; RIKEN Preventive Medicine and Diagnosis Innovation Program, Japan.
  • Takasaki T; Kanagawa Prefectural Institute of Public Health, Japan.
Jpn J Infect Dis ; 75(3): 277-280, 2022 May 24.
Article in English | MEDLINE | ID: covidwho-1865649
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China, in December 2019. Despite the recent introduction of vaccines against SARS-CoV-2, more effective vaccines and antiviral drugs must be developed. Here, we isolated five SARS-CoV-2 strains from four patients with coronavirus disease (COVID-19) and an asymptomatic individual using pharyngeal swabs, nasopharyngeal swabs, and sputum samples. Cytopathic effects in inoculated Vero cells were observed between days 3 and 7. SARS-CoV-2 infection was confirmed by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) and next-generation sequencing. Phylogenetic analyses of the whole genome sequences showed that the virus isolates from the clinical samples belonged to the Wuhan and European lineages. These findings and the isolated viruses may contribute to the development of diagnostic tools, vaccines, and antiviral drugs for COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Language: English Journal: Jpn J Infect Dis Journal subject: Communicable Diseases Year: 2022 Document Type: Article Affiliation country: Yoken.JJID.2021.292

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Language: English Journal: Jpn J Infect Dis Journal subject: Communicable Diseases Year: 2022 Document Type: Article Affiliation country: Yoken.JJID.2021.292