In vitro evaluation of antiviral activity of Sisybrium irio (Khaksi) against SARS-CoV-2
Pakistan Journal of Pharmaceutical Sciences
; 35(3):859-864, 2022.
Article
in English
| CAB Abstracts | ID: covidwho-1865773
ABSTRACT
SARS-CoV-2 pandemic, drawn attention to the need of virus culture. In vitro SARS-COV-2 culture was performed to carry out therapeutic, environmental and virus genome studies. Isolation of virus from nasopharyngeal swab was performed by inoculating virus positive samples in available cell lines. SARS-CoV-2 topography was observed by using Scanning Electron Microscopy (SEM). Virus specificity was defined by serological confirmation through neutralization assay with COVID 19 convalescent sera. The SARS-COV -2 virus replicated successfully in Vero cell lines (both in E6 and CCL-81). The TCID50 and PFUs of isolated virus were defined as 107 TCID50/mL and 1.4 x 106 pfu/mL respectively. The virus particles with the SARS-CoV morphology was 150 engM size. Virus inhibition in presence of convalescent sera of COVID-19 patients was observed. Sisybrium irio (Khaksi) was found cytotoxic on Vero E6 cell line and its antiviral activity could not be established against SARS-COV-2 virus in vitro. Successful isolated and archived native SARS-COV-2 may be utilized further for therapeutic, environmental and virus genome sequencing studies.
Prion, Viral, Bacterial and Fungal Pathogens of Humans [VV210]; Non-food/Non-feed Plant Products [SS200]; Animal and in vitro Models for Pharmaceuticals [VV450]; Pesticides and Drugs; Control [HH405]; in vitro; antiviral properties; cell lines; genomes; nucleotide sequences; pathogens; viral replication; particle size; medicinal plants; cytotoxicity; Severe acute respiratory syndrome coronavirus 2; Sisymbrium irio; plants; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Sisymbrium; Brassicaceae; Brassicales; eudicots; angiosperms; Spermatophyta; eukaryotes; SARS-CoV-2; anti-viral properties; DNA sequences; drug plants; medicinal herbs; officinal plants
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Type of study:
Experimental Studies
Language:
English
Journal:
Pakistan Journal of Pharmaceutical Sciences
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS