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Binding of SARS-CoV-2 protein ORF9b to mitochondrial translocase TOM70 prevents its interaction with chaperone HSP90.
Ayinde, Kehinde S; Pinheiro, Glaucia M S; Ramos, Carlos H I.
  • Ayinde KS; Institute of Chemistry, University of Campinas UNICAMP, 13083-970, Campinas, SP, Brazil; Institute of Biology, University of Campinas (UNICAMP), SP, Brazil.
  • Pinheiro GMS; Institute of Chemistry, University of Campinas UNICAMP, 13083-970, Campinas, SP, Brazil.
  • Ramos CHI; Institute of Chemistry, University of Campinas UNICAMP, 13083-970, Campinas, SP, Brazil. Electronic address: cramos@unicamp.br.
Biochimie ; 200: 99-106, 2022 Sep.
Article in English | MEDLINE | ID: covidwho-1866907
ABSTRACT
The emergence of the COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains a great threat to global health. ORF9b, an important accessory protein of SARS-CoV-2, plays a critical role in the viral host interaction, targeting TOM70, a member of the mitochondrial translocase of the outer membrane complex. The assembly between ORF9b and TOM70 is implicated in disrupting mitochondrial antiviral signaling, leading to immune evasion. We describe the expression, purification, and characterization of ORF9b alone or coexpressed with the cytosolic domain of human TOM70 in E. coli. ORF9b has 97 residues and was purified as a homodimer with an molecular mass of 22 kDa as determined by SEC-MALS. Circular dichroism experiments showed that Orf9b alone exhibits a random conformation. The ORF9b-TOM70 complex characterized by CD and differential scanning calorimetry showed that the complex is folded and more thermally stable than free TOM70, indicating strong binding. Importantly, protein-protein interaction assays demonstrated that full-length human Hsp90 is capable of binding to free TOM70 but not to the ORF9b-TOM70 complex. To narrow down the nature of this inhibition, the isolated C-terminal domain of Hsp90 was also tested. These results were used to build a model of the mechanism of inhibition, in which ORF9b efficiently targets two sites of interaction between TOM70 and Hsp90. The findings showed that ORF9b complexed with TOM70 prevents the interaction with Hsp90, and this is one major explanation for SARS-CoV-2 evasion of host innate immunity via the inhibition of the interferon activation pathway.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Randomized controlled trials Limits: Humans Language: English Journal: Biochimie Year: 2022 Document Type: Article Affiliation country: J.biochi.2022.05.016

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Randomized controlled trials Limits: Humans Language: English Journal: Biochimie Year: 2022 Document Type: Article Affiliation country: J.biochi.2022.05.016