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Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoprotein.
Tortorici, M Alejandra; Walls, Alexandra C; Joshi, Anshu; Park, Young-Jun; Eguia, Rachel T; Miranda, Marcos C; Kepl, Elizabeth; Dosey, Annie; Stevens-Ayers, Terry; Boeckh, Michael J; Telenti, Amalio; Lanzavecchia, Antonio; King, Neil P; Corti, Davide; Bloom, Jesse D; Veesler, David.
  • Tortorici MA; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
  • Walls AC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
  • Joshi A; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
  • Park YJ; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
  • Eguia RT; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
  • Miranda MC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.
  • Kepl E; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.
  • Dosey A; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.
  • Stevens-Ayers T; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
  • Boeckh MJ; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
  • Telenti A; Vir Biotechnology, San Francisco, CA 94158, USA.
  • Lanzavecchia A; Istituto Nazionale Genetica Molecolare, 20122 Milano, Italy; Humabs Biomed SA-a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.
  • King NP; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.
  • Corti D; Humabs Biomed SA-a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.
  • Bloom JD; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
  • Veesler D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA. Electronic address: dveesler@uw.edu.
Cell ; 185(13): 2279-2291.e17, 2022 06 23.
Article in English | MEDLINE | ID: covidwho-1866951
ABSTRACT
The isolation of CCoV-HuPn-2018 from a child respiratory swab indicates that more coronaviruses are spilling over to humans than previously appreciated. We determined the structures of the CCoV-HuPn-2018 spike glycoprotein trimer in two distinct conformational states and showed that its domain 0 recognizes sialosides. We identified that the CCoV-HuPn-2018 spike binds canine, feline, and porcine aminopeptidase N (APN) orthologs, which serve as entry receptors, and determined the structure of the receptor-binding B domain in complex with canine APN. The introduction of an oligosaccharide at position N739 of human APN renders cells susceptible to CCoV-HuPn-2018 spike-mediated entry, suggesting that single-nucleotide polymorphisms might account for viral detection in some individuals. Human polyclonal plasma antibodies elicited by HCoV-229E infection and a porcine coronavirus monoclonal antibody inhibit CCoV-HuPn-2018 spike-mediated entry, underscoring the cross-neutralizing activity among ɑ-coronaviruses. These data pave the way for vaccine and therapeutic development targeting this zoonotic pathogen representing the eighth human-infecting coronavirus.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus Infections / Coronavirus / Coronavirus 229E, Human Type of study: Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Language: English Journal: Cell Year: 2022 Document Type: Article Affiliation country: J.cell.2022.05.019

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus Infections / Coronavirus / Coronavirus 229E, Human Type of study: Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Language: English Journal: Cell Year: 2022 Document Type: Article Affiliation country: J.cell.2022.05.019