Heterogeneity of In Vitro Expanded Mesenchymal Stromal Cells and Strategies to Improve Their Therapeutic Actions
Pharmaceutics
; 14(5):1112, 2022.
Article
in English
| ProQuest Central | ID: covidwho-1871481
ABSTRACT
Beneficial properties of mesenchymal stromal cells (MSCs) have prompted their use in preclinical and clinical research. Accumulating evidence has been provided for the therapeutic effects of MSCs in several pathologies, including neurodegenerative diseases, myocardial infarction, skin problems, liver disorders and cancer, among others. Although MSCs are found in multiple tissues, the number of MSCs is low, making in vitro expansion a required step before MSC application. However, culture-expanded MSCs exhibit notable differences in terms of cell morphology, physiology and function, which decisively contribute to MSC heterogeneity. The changes induced in MSCs during in vitro expansion may account for the variability in the results obtained in different MSC-based therapy studies, including those using MSCs as living drug delivery systems. This review dissects the different changes that occur in culture-expanded MSCs and how these modifications alter their therapeutic properties after transplantation. Furthermore, we discuss the current strategies developed to improve the beneficial effects of MSCs for successful clinical implementation, as well as potential therapeutic alternatives.
Pharmacy And Pharmacology; mesenchymal stem cells; cell therapy; regenerative medicine; cell engineering; drug delivery; preconditioning; scaffolds; secretome; induced pluripotent stem cells; Body fat; Umbilical cord; Diabetes; Bone marrow; Antigens; Severe acute respiratory syndrome coronavirus 2; Adipocytes; Morphology; Dental pulp; Protein expression; Hypoxia; Proteins
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Collection:
Databases of international organizations
Database:
ProQuest Central
Language:
English
Journal:
Pharmaceutics
Year:
2022
Document Type:
Article
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