The Viability of Hematopoietic Progenitor Cell Grafts after Cryopreservation Does Not Predict Delayed Engraftment in Allogeneic Hematopoietic Stem Cell Transplantation.
Lab Med
; 53(5): 509-513, 2022 Sep 01.
Article
in English
| MEDLINE | ID: covidwho-1873968
ABSTRACT
OBJECTIVE:
Due to the COVID-19 pandemic, more peripheral blood stem cell (PBSC) allogeneic grafts are being frozen and infused thawed. Our objective was to study the influence of graft viability on engraftment outcome in patients treated with PBSCs.METHODS:
Using trypan blue stain, we compared total nucleated cell (TNC) viability of both fresh and thawed grafts in allogeneic PBSCs.RESULTS:
The viability of thawed PBSC grafts median was 74%, and fresh was 99.0%. The median number of CD34â +â cells/kg infused thawed was 6.3â ×â 106/kg and median time to neutrophil and platelet engraftment was 17.5 and 20 days. Median number of CD34â +â cells/kg infused fresh was 5.1â ×â 106/kg and median time to neutrophil and platelet engraftment was 18 and 19 days. There were no statistically significant differences in the time to engraftment between the 2 groups.CONCLUSION:
A low TNC viability of thawed PBSC grafts does not have an effect on time to neutrophil and platelet engraftment when more than 2.85â ×â 106 CD34â +â cells/kg are infused.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Hematopoietic Stem Cell Transplantation
/
COVID-19
Type of study:
Experimental Studies
/
Prognostic study
/
Randomized controlled trials
Limits:
Humans
Language:
English
Journal:
Lab Med
Year:
2022
Document Type:
Article
Affiliation country:
Labmed
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