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Phenotypic Adaptation to Antiseptics and Effects on Biofilm Formation Capacity and Antibiotic Resistance in Clinical Isolates of Early Colonizers in Dental Plaque.
Auer, David L; Mao, Xiaojun; Anderson, Annette Carola; Muehler, Denise; Wittmer, Annette; von Ohle, Christiane; Wolff, Diana; Frese, Cornelia; Hiller, Karl-Anton; Maisch, Tim; Buchalla, Wolfgang; Hellwig, Elmar; Al-Ahmad, Ali; Cieplik, Fabian.
  • Auer DL; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, Germany.
  • Mao X; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, Germany.
  • Anderson AC; Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Faculty of Medicine, University of Freiburg, 79085 Freiburg im Breisgau, Germany.
  • Muehler D; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, Germany.
  • Wittmer A; Institute of Medical Microbiology and Hygiene, Faculty of Medicine, University of Freiburg, 79085 Freiburg im Breisgau, Germany.
  • von Ohle C; Department of Conservative Dentistry, Periodontology and Endodontology, University Hospital Tübingen, 72076 Tübingen, Germany.
  • Wolff D; Department of Conservative Dentistry, University Hospital Heidelberg, 69120 Heidelberg, Germany.
  • Frese C; Department of Conservative Dentistry, University Hospital Heidelberg, 69120 Heidelberg, Germany.
  • Hiller KA; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, Germany.
  • Maisch T; Department of Dermatology, University Hospital Regensburg, 93053 Regensburg, Germany.
  • Buchalla W; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, Germany.
  • Hellwig E; Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Faculty of Medicine, University of Freiburg, 79085 Freiburg im Breisgau, Germany.
  • Al-Ahmad A; Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Faculty of Medicine, University of Freiburg, 79085 Freiburg im Breisgau, Germany.
  • Cieplik F; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, Germany.
Antibiotics (Basel) ; 11(5)2022 May 19.
Article in English | MEDLINE | ID: covidwho-1875459
ABSTRACT
Despite the wide-spread use of antiseptics in dental practice and oral care products, there is little public awareness of potential risks associated with antiseptic resistance and potentially concomitant cross-resistance. Therefore, the aim of this study was to investigate potential phenotypic adaptation in 177 clinical isolates of early colonizers of dental plaque (Streptococcus, Actinomyces, Rothia and Veillonella spp.) upon repeated exposure to subinhibitory concentrations of chlorhexidine digluconate (CHX) or cetylpyridinium chloride (CPC) over 10 passages using a modified microdilution method. Stability of phenotypic adaptation was re-evaluated after culture in antiseptic-free nutrient broth for 24 or 72 h. Strains showing 8-fold minimal inhibitory concentration (MIC)-increase were further examined regarding their biofilm formation capacity, phenotypic antibiotic resistance and presence of antibiotic resistance genes (ARGs). Eight-fold MIC-increases to CHX were detected in four Streptococcus isolates. These strains mostly exhibited significantly increased biofilm formation capacity compared to their respective wild-type strains. Phenotypic antibiotic resistance was detected to tetracycline and erythromycin, consistent with the detected ARGs. In conclusion, this study shows that clinical isolates of early colonizers of dental plaque can phenotypically adapt toward antiseptics such as CHX upon repeated exposure. The underlying mechanisms at genomic and transcriptomic levels need to be investigated in future studies.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Language: English Year: 2022 Document Type: Article Affiliation country: Antibiotics11050688

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Language: English Year: 2022 Document Type: Article Affiliation country: Antibiotics11050688