Random Copolymers of Lysine and Isoleucine for Efficient mRNA Delivery.
Int J Mol Sci
; 23(10)2022 May 11.
Article
in English
| MEDLINE | ID: covidwho-1875639
ABSTRACT
Messenger RNA (mRNA) is currently of great interest as a new category of therapeutic agent, which could be used for prevention or treatment of various diseases. For this mRNA requires effective delivery systems that will protect it from degradation, as well as allow cellular uptake and mRNA release. Random poly(lysine-co-isoleucine) polypeptides were synthesized and investigated as possible carriers for mRNA delivery. The polypeptides obtained under lysineisoleucine monomer ratio equal to 80/20 were shown to give polyplexes with smaller size, positive ζ-potential and more than 90% encapsulation efficacy. The phase inversion method was proposed as best way for encapsulation of mRNA into polyplexes, which are based on obtained amphiphilic copolymers. These copolymers showed efficacy in protection of bound mRNA towards ribonuclease and lower toxicity as compared to lysine homopolymer. The poly(lysine-co-isoleucine) polypeptides showed greater than poly(ethyleneimine) efficacy as vectors for transfection of cells with green fluorescent protein and firefly luciferase encoding mRNAs. This allows us to consider obtained copolymers as promising candidates for mRNA delivery applications.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Isoleucine
/
Lysine
Type of study:
Randomized controlled trials
Language:
English
Year:
2022
Document Type:
Article
Affiliation country:
Ijms23105363
Similar
MEDLINE
...
LILACS
LIS