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Monitoring SARS-CoV-2 in the Wastewater and Rivers of Tapachula, a Migratory Hub in Southern Mexico.
Zarza, Eugenia; Diego-García, Elia; García, Luz Verónica; Castro, Ricardo; Mejía, Gamaliel; Herrera, David; Cuevas, Raúl; Palomeque, Ángeles; Isa, Pavel; Guillén, Karina.
  • Zarza E; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Diego-García E; Investigadoras CONACyT- El Colegio de la Frontera Sur, Av. Insurgentes Sur 1582, Col. Crédito Constructor, Benito Juárez, 03940, Mexico City, Mexico.
  • García LV; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Castro R; Investigadoras CONACyT- El Colegio de la Frontera Sur, Av. Insurgentes Sur 1582, Col. Crédito Constructor, Benito Juárez, 03940, Mexico City, Mexico.
  • Mejía G; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Herrera D; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Cuevas R; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Palomeque Á; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Isa P; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Guillén K; El Colegio de la Frontera Sur (ECOSUR), Grupo Académico de Biotecnología Ambiental, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
Food Environ Virol ; 14(2): 199-211, 2022 06.
Article in English | MEDLINE | ID: covidwho-1877976
ABSTRACT
The COVID-19 pandemic has been monitored by applying different strategies, including SARS-CoV-2 detection with clinical testing or through wastewater-based epidemiology (WBE). We used the latter approach to follow SARS-CoV-2 dispersion in Tapachula city, located in Mexico's tropical southern border region. Tapachula is a dynamic entry point for people seeking asylum in Mexico or traveling to the USA. Clinical testing facilities for SARS-CoV-2 monitoring are limited in the city. A total of eighty water samples were collected from urban and suburban rivers and sewage and a wastewater treatment plant over 4 months in Tapachula. We concentrated viral particles with a PEG-8000-based method, performed RNA extraction, and detected SARS-CoV-2 particles through RT-PCR. We considered the pepper mild mottle virus as a fecal water pollution biomarker and analytical control. SARS-CoV-2 viral loads (N1 and N2 markers) were quantified and correlated with official regional statistics of COVID-19 bed occupancy and confirmed cases (r > 91%). Our results concluded that WBE proved a valuable tool for tracing and tracking the COVID-19 pandemic in tropical countries with similar water temperatures (21-29 °C). Monitoring SARS-CoV-2 through urban and suburban river water sampling would be helpful in places lacking a wastewater treatment plant or water bodies with sewage discharges.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Observational study / Prognostic study Limits: Humans Country/Region as subject: Mexico Language: English Journal: Food Environ Virol Year: 2022 Document Type: Article Affiliation country: S12560-022-09523-2

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Observational study / Prognostic study Limits: Humans Country/Region as subject: Mexico Language: English Journal: Food Environ Virol Year: 2022 Document Type: Article Affiliation country: S12560-022-09523-2