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CD66b-CD64dimCD115- cells in the human bone marrow represent neutrophil-committed progenitors.
Calzetti, Federica; Finotti, Giulia; Tamassia, Nicola; Bianchetto-Aguilera, Francisco; Castellucci, Monica; Canè, Stefania; Lonardi, Silvia; Cavallini, Chiara; Matte, Alessandro; Gasperini, Sara; Signoretto, Ilaria; Benedetti, Fabio; Bonifacio, Massimiliano; Vermi, William; Ugel, Stefano; Bronte, Vincenzo; Tecchio, Cristina; Scapini, Patrizia; Cassatella, Marco A.
  • Calzetti F; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
  • Finotti G; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
  • Tamassia N; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
  • Bianchetto-Aguilera F; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
  • Castellucci M; Centro Piattaforme Tecnologiche, University of Verona, Verona, Italy.
  • Canè S; Section of Immunology, Department of Medicine, University of Verona, Verona, Italy.
  • Lonardi S; Unit of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
  • Cavallini C; Interdepartmental Laboratory of Medical Research, Research Center LURM, University of Verona, Verona, Italy.
  • Matte A; Section of Internal Medicine B, Department of Medicine, University of Verona and AOUI Verona, Verona, Italy.
  • Gasperini S; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
  • Signoretto I; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
  • Benedetti F; Section of Hematology and Bone Marrow Transplant Unit, Department of Medicine, University of Verona, Verona, Italy.
  • Bonifacio M; Section of Hematology and Bone Marrow Transplant Unit, Department of Medicine, University of Verona, Verona, Italy.
  • Vermi W; Unit of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
  • Ugel S; Section of Immunology, Department of Medicine, University of Verona, Verona, Italy.
  • Bronte V; Section of Immunology, Department of Medicine, University of Verona, Verona, Italy.
  • Tecchio C; Section of Hematology and Bone Marrow Transplant Unit, Department of Medicine, University of Verona, Verona, Italy.
  • Scapini P; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy.
  • Cassatella MA; Section of General Pathology, Department of Medicine, University of Verona, Verona, Italy. marco.cassatella@univr.it.
Nat Immunol ; 23(5): 679-691, 2022 05.
Article in English | MEDLINE | ID: covidwho-1878539
ABSTRACT
Here we report the identification of human CD66b-CD64dimCD115- neutrophil-committed progenitor cells (NCPs) within the SSCloCD45dimCD34+ and CD34dim/- subsets in the bone marrow. NCPs were either CD45RA+ or CD45RA-, and in vitro experiments showed that CD45RA acquisition was not mandatory for their maturation process. NCPs exclusively generated human CD66b+ neutrophils in both in vitro differentiation and in vivo adoptive transfer experiments. Single-cell RNA-sequencing analysis indicated NCPs fell into four clusters, characterized by different maturation stages and distributed along two differentiation routes. One of the clusters was characterized by an interferon-stimulated gene signature, consistent with the reported expansion of peripheral mature neutrophil subsets that express interferon-stimulated genes in diseased individuals. Finally, comparison of transcriptomic and phenotypic profiles indicated NCPs represented earlier neutrophil precursors than the previously described early neutrophil progenitors (eNePs), proNeus and COVID-19 proNeus. Altogether, our data shed light on the very early phases of neutrophil ontogeny.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bone Marrow / Antigens, CD / Cell Adhesion Molecules / Cell Differentiation / Receptors, IgG / Receptor, Macrophage Colony-Stimulating Factor / Neutrophils Limits: Humans Language: English Journal: Nat Immunol Journal subject: Allergy and Immunology Year: 2022 Document Type: Article Affiliation country: S41590-022-01189-z

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bone Marrow / Antigens, CD / Cell Adhesion Molecules / Cell Differentiation / Receptors, IgG / Receptor, Macrophage Colony-Stimulating Factor / Neutrophils Limits: Humans Language: English Journal: Nat Immunol Journal subject: Allergy and Immunology Year: 2022 Document Type: Article Affiliation country: S41590-022-01189-z