CD66b-CD64dimCD115- cells in the human bone marrow represent neutrophil-committed progenitors.
Nat Immunol
; 23(5): 679-691, 2022 05.
Article
in English
| MEDLINE | ID: covidwho-1878539
ABSTRACT
Here we report the identification of human CD66b-CD64dimCD115- neutrophil-committed progenitor cells (NCPs) within the SSCloCD45dimCD34+ and CD34dim/- subsets in the bone marrow. NCPs were either CD45RA+ or CD45RA-, and in vitro experiments showed that CD45RA acquisition was not mandatory for their maturation process. NCPs exclusively generated human CD66b+ neutrophils in both in vitro differentiation and in vivo adoptive transfer experiments. Single-cell RNA-sequencing analysis indicated NCPs fell into four clusters, characterized by different maturation stages and distributed along two differentiation routes. One of the clusters was characterized by an interferon-stimulated gene signature, consistent with the reported expansion of peripheral mature neutrophil subsets that express interferon-stimulated genes in diseased individuals. Finally, comparison of transcriptomic and phenotypic profiles indicated NCPs represented earlier neutrophil precursors than the previously described early neutrophil progenitors (eNePs), proNeus and COVID-19 proNeus. Altogether, our data shed light on the very early phases of neutrophil ontogeny.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Bone Marrow
/
Antigens, CD
/
Cell Adhesion Molecules
/
Cell Differentiation
/
Receptors, IgG
/
Receptor, Macrophage Colony-Stimulating Factor
/
Neutrophils
Limits:
Humans
Language:
English
Journal:
Nat Immunol
Journal subject:
Allergy and Immunology
Year:
2022
Document Type:
Article
Affiliation country:
S41590-022-01189-z
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