Correlation between real-time PCR cycle threshold and clinical classification of COVID-19
Revista Chilena De Infectologia
; 39(1):35-44, 2022.
Article
in Spanish
| Web of Science Web of Science | ID: covidwho-1880752
ABSTRACT
Background:
The cycle threshold (Ct) of real-time reverse transcription PCR (RT-qPCR) indicates the relative concentration of an RNA sequence, this value has been related to clinical profile in viral infections.Aim:
To determine the correlation between the Ct value and the clinical classification of COVID-19.Method:
A correlational cross-sectional study was carried out, the Ct values were obtained by RT-qPCR directed to the N gene of SARS-CoV-2, grouping them by means of a central robust estimator and related to the clinical classification of COVID-19.Results:
Of the 718 cases included in the study;77.7% (558) were mild;21.3% (153) moderate and 1% (7) severe. The Ct value was grouped into levels low Ct <= 18.83;medium Ct> 18.83- 30.10 and high Ct> 30.10. There was a weak inverse significant correlation (p = 0.002;Spearman's rho = -0.117) between the Ct value and the clinical classification. The characteristics sex, age under 65 years, fever, chills, diarrhea, anosmia, and overweight-obesity were associated with the Ct value.Conclusion:
The lower the Ct value, a classification of greater severity of COVID-19 is expected, however, because the correlation is weak, its usefulness as a severity predictor is limited.
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Collection:
Databases of international organizations
Database:
Web of Science Web of Science
Type of study:
Prognostic study
Language:
Spanish
Journal:
Revista Chilena De Infectologia
Year:
2022
Document Type:
Article
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