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New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors.
Wille, Diana; Heinzelmann, Joana; Kehlen, Astrid; Lütgehetmann, Marc; Nörz, Dominik S; Siebolts, Udo; Mueller, Anke; Karrasch, Matthias; Hofmann, Nicola; Viestenz, Anja; Börgel, Martin; Kuhn, Ferenc; Viestenz, Arne.
  • Wille D; Department of Ophthalmology, Halle, University Hospital Halle-Wittenberg, 06120 Halle, Germany.
  • Heinzelmann J; Mitteldeutsche Cornebank Halle, University Hospital Halle-Wittenberg, 06120 Halle, Germany.
  • Kehlen A; German Society for Tissue Transplantation (DGFG) gGmbH, 30625 Hannover, Germany.
  • Lütgehetmann M; Department of Ophthalmology, Halle, University Hospital Halle-Wittenberg, 06120 Halle, Germany.
  • Nörz DS; Department for Laboratory Medicine, Unit III, Molecular Diagnostic Section, University Hospital Halle-Wittenberg, 06120 Halle, Germany.
  • Siebolts U; Institute for Medical Microbiology, Virology and Hygiene, University Hospital Hamburg-Eppendorf, 20246 Hamburg, Germany.
  • Mueller A; Institute for Medical Microbiology, Virology and Hygiene, University Hospital Hamburg-Eppendorf, 20246 Hamburg, Germany.
  • Karrasch M; Institute of Pathology, University Hospital Halle-Wittenberg, 06112 Halle, Germany.
  • Hofmann N; Institute for Medical Microbiology, University Hospital Halle-Wittenberg, 06112 Halle, Germany.
  • Viestenz A; Department for Laboratory Medicine, Unit III, Molecular Diagnostic Section, University Hospital Halle-Wittenberg, 06120 Halle, Germany.
  • Börgel M; German Society for Tissue Transplantation (DGFG) gGmbH, 30625 Hannover, Germany.
  • Kuhn F; Department of Ophthalmology, Halle, University Hospital Halle-Wittenberg, 06120 Halle, Germany.
  • Viestenz A; German Society for Tissue Transplantation (DGFG) gGmbH, 30625 Hannover, Germany.
J Clin Med ; 11(12)2022 Jun 09.
Article in English | MEDLINE | ID: covidwho-1884245
ABSTRACT
In the tissue donation field, to prevent pathogen transmission, all donors are screened by postmortem swabs for SARS-CoV-2 using qRT-PCR. Corneas from donors who tested positive for SARS-CoV-2 were subjected to further investigations. Corneal transplants and culture medium from positive donors were cultured under appropriate safety conditions for further analyses. Cornea tissue samples, including sclera/limbus/cornea, and culture media were taken at different time points for testing for SARS-CoV-2 using qRT-PCR, immunohistochemistry (IHC) and subgenomic RNA (sgRNA) analysis. Between January and May 2021, in four donors with initial negative premortem rapid tests, SARS-CoV-2 was detected post-mortem using qRT-PCR. In these cases, SARS-CoV-2 was observed at the beginning of cultivation in both tissue and culture medium using qRT-PCR and IHC. The virus was mainly localized in the limbus epithelial cells, with a stable detection level. Premortem rapid tests are potentially insufficient to exclude SARS-CoV-2 infection in corneal donors. While, for SARS-CoV-2, the risk of infection via transplants is considered low, a residual risk remains for presymptomatic new infections. However, our investigations provide the first indications that, with organ cultures, the risk of virus transmission is minimized due to the longer minimum culture period.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Year: 2022 Document Type: Article Affiliation country: Jcm11123312

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Year: 2022 Document Type: Article Affiliation country: Jcm11123312