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Antibodies targeting conserved non-canonical antigens and endemic coronaviruses associate with favorable outcomes in severe COVID-19.
Peddireddy, Sai Preetham; Rahman, Syed A; Cillo, Anthony R; Vijay, Godhev Manakkat; Somasundaram, Ashwin; Workman, Creg J; Bain, William; McVerry, Bryan J; Methe, Barbara; Lee, Janet S; Ray, Prabir; Ray, Anuradha; Bruno, Tullia C; Vignali, Dario A A; Kitsios, Georgios D; Morris, Alison; Singh, Harinder; Sarkar, Aniruddh; Das, Jishnu.
  • Peddireddy SP; Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
  • Rahman SA; Center for Systems Immunology, Departments of Immunology and Computational & Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA.
  • Cillo AR; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
  • Vijay GM; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
  • Somasundaram A; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
  • Workman CJ; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
  • Bain W; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • McVerry BJ; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Methe B; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Lee JS; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Ray P; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Ray A; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Bruno TC; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
  • Vignali DAA; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
  • Kitsios GD; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Morris A; Division of Pulmonary Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Singh H; Center for Systems Immunology, Departments of Immunology and Computational & Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: harinder@pitt.edu.
  • Sarkar A; Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA. Electronic address: aniruddh.sarkar@bme.gatech.edu.
  • Das J; Center for Systems Immunology, Departments of Immunology and Computational & Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: jishnu@pitt.edu.
Cell Rep ; 39(13): 111020, 2022 06 28.
Article in English | MEDLINE | ID: covidwho-1885675
ABSTRACT
While there have been extensive analyses characterizing cellular and humoral responses across the severity spectrum in COVID-19, outcome predictors within severe COVID-19 remain less comprehensively elucidated. Furthermore, properties of antibodies (Abs) directed against viral antigens beyond spike and their associations with disease outcomes remain poorly defined. We perform deep molecular profiling of Abs directed against a wide range of antigenic specificities in severe COVID-19 patients. The profiles included canonical (spike [S], receptor-binding domain [RBD], and nucleocapsid [N]) and non-canonical (orf3a, orf8, nsp3, nsp13, and membrane [M]) antigenic specificities. Notably, multivariate Ab profiles directed against canonical or non-canonical antigens are equally discriminative of survival in severe COVID-19. Intriguingly, pre-pandemic healthy controls have cross-reactive Abs directed against nsp13, a protein conserved across coronaviruses. Consistent with these findings, a model built on Ab profiles for endemic coronavirus antigens also predicts COVID-19 outcome. Our results suggest the importance of studying Abs targeting non-canonical severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and endemic coronavirus antigens in COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Cell Rep Year: 2022 Document Type: Article Affiliation country: J.celrep.2022.111020

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Cell Rep Year: 2022 Document Type: Article Affiliation country: J.celrep.2022.111020