ULTRASTRUCTURAL FINDINGS OF COVID-19 INFECTION IN THE PROSTATE
Journal of Urology
; 207(SUPPL 5):e269-e270, 2022.
Article
in English
| EMBASE | ID: covidwho-1886491
ABSTRACT
INTRODUCTION AND OBJECTIVE:
SARS-CoV-2 utilizes two integral membrane proteins ACE2 and TMPRSS2 for viral replication. It has been established TMPRS22 specifically is found in high concentrations throughout the prostate found to be linked to prostatic disease progression. This project examined the histopathological, ultrastructural, and immunofluorescent elements of prostatic tissue from men infected by SARS-CoV-2.METHODS:
We evaluated prostate tissue in men with worsening lower urinary tract symptoms who underwent HoLEP procedure after SARS-CoV-2 infection. Biopsied tissue was visualized by transmission electron microscopy (TEM), immunofluorescence, and viral presence was confirmed by quantitative polymerase chain reaction (RT-PCR).RESULTS:
Multiple coronavirus-like spiked viral particles ranging from 73.3mm to 109mm were visualized by TEM (Figure). Histochemical and immunofluorescence concurrently showed presence of distinct hyalinization, fibrosis, and presence of spike protein (Figure 2). RT-PCR confirmed the identity of the viral bodies as SARS-CoV-2 (Figure 3).CONCLUSIONS:
This study provides evidence that SARSCoV- 2 not only enters prostatic tissue but may persist beyond initial infection period. In addition to establishing the persistence of SARSCoV- 2 particles in prostatic tissue, this report suggests the importance of discerning the relationships between COVID-19, lower urinary tract symptom severity, and prostatic hyperplasia. (Figure Presented).
endogenous compound; virus spike protein; adult; conference abstract; controlled study; coronavirus disease 2019; fibrosis; histopathology; human; human tissue; hyaline degeneration; immunofluorescence; lower urinary tract symptom; male; nonhuman; prostate hypertrophy; prostate tissue; prostatitis; quantitative analysis; real time polymerase chain reaction; Severe acute respiratory syndrome coronavirus 2; transmission electron microscopy; virus particle
Full text:
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Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Journal of Urology
Year:
2022
Document Type:
Article
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