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Increased proinflammatory cytokines in tears correspond with conjunctival SARS-CoV-2 positivity in symptomatic COVID-19 patients.
Niedzwiedz, Anna; Kawa, Milosz; Pius-Sadowska, Ewa; Kuligowska, Agnieszka; Ziontkowska, Alicja; Wrzalek, Dawid; Wiacek, Marta P; Parczewski, Milosz; Ossowski, Andrzej; Zielinska, Grazyna; Safranow, Krzysztof; Kozlowski, Krzysztof; Machalinski, Boguslaw; Machalinska, Anna.
  • Niedzwiedz A; Department of General Pathology, Pomeranian Medical University in Szczecin, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Kawa M; Department of General Pathology, Pomeranian Medical University in Szczecin, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Pius-Sadowska E; Department of General Pathology, Pomeranian Medical University in Szczecin, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Kuligowska A; First Department of Ophthalmology, Pomeranian Medical University, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Ziontkowska A; First Department of Ophthalmology, Pomeranian Medical University, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Wrzalek D; First Department of Ophthalmology, Pomeranian Medical University, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Wiacek MP; First Department of Ophthalmology, Pomeranian Medical University, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Parczewski M; Department of Infectious, Tropical Diseases and Immune Deficiency, Pomeranian Medical University in Szczecin, Arkonska 4 Street, 71-455, Szczecin, Poland.
  • Ossowski A; Department of Forensic Genetics, Pomeranian Medical University in Szczecin, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Zielinska G; Department of Forensic Genetics, Pomeranian Medical University in Szczecin, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Safranow K; Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Kozlowski K; Department of Constitutional Law, Faculty of Law and Administration of the Jagiellonian University in Krakow, Bracka 12 Street, 31-005, Kraków, Poland.
  • Machalinski B; Department of General Pathology, Pomeranian Medical University in Szczecin, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland.
  • Machalinska A; First Department of Ophthalmology, Pomeranian Medical University, Al. Powstanców Wielkopolskich 72, 70-111, Szczecin, Poland. anna.machalinska@pum.edu.pl.
Sci Rep ; 12(1): 7225, 2022 05 04.
Article in English | MEDLINE | ID: covidwho-1890252
ABSTRACT
Tear fluid cytokine levels may serve as biomarkers of innate immune system response against SARS-CoV-2 infection. Therefore, our aim was to analyze panel of selected inflammatory cytokines in tears of COVID-19 patients in relation to presence of SARS-CoV-2 viral load in conjunctival secretions. In this study concentrations of TNF-α, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 p70, GM-CSF, and IFN-γ were determined by a magnetic bead assay in tear film collected from 232 symptomatic COVID-19 patients. SARS-CoV-2 ocular infection was confirmed based on positive conjunctival swab-based RT-PCR testing. Viral RNA in conjunctival sac was detected in 21 patients (9%). No relation between presence and the duration of ophthalmic symptoms and SARS-CoV-2 infection detected in conjunctival secretions was found. The tear film concentrations of IFN-γ, TNF-α, IL-5, IL-8 and GM-CSF were found to be significantly greater among patients with positive conjunctival swab results as compared to the group negative for SARS-CoV-2 in conjunctival sac. Our current data depict a group of inflammatory mediators in human tears, which may play a significant role in ocular pathology of SARS-CoV-2 conjunctival infection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-11285-7

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-11285-7