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Oral saliva swab reverse transcription PCR for Covid-19 in the paediatric population.
Moraleda, Cinta; Domínguez-Rodríguez, Sara; Mesa, Juan Miguel; García-Sánchez, Paula; de la Serna, María; Alonso-Cadenas, José Antonio; Bermejo, Amanda; Sabrido, Gema; Martínez-Campos, Leticia; González-Posada, Aránzazu Flavia; Illán-Ramos, Marta; Cobos-Carrascosa, Elena; Ballesteros, Álvaro; Galán, Juan Carlos; Llorente, Francisco; Aguilera-Alonso, David; Jiménez, Ana Belén; Catalán, Pilar; Dahdouh, Elias; Navarro, Ignacio; Fernández-Garoz, Bárbara; Mendoza, Pablo; Pérez-Jorge, Concepción; Cabezas-Fernández, Teresa; Blázquez-Gamero, Daniel; Rivas, Gonzalo; Gonzalez-Donapetry, Patricia; Sáez, Elena; Fernández-Pinero, Jovita; Lucas-Fernández, Jesús; Pérez-Ramírez, Elisa; Merino, Paloma; Miragaya, Sandra; Lorente, Jorge; Iglesias, Irene; Tagarro, Alfredo.
  • Moraleda C; Pediatric Infectious Diseases Unit. Department of Pediatrics, Hospital Universitario 12 de Octubre. Pediatric Research and Clinical Trials Unit (UPIC). RITIP (Translational Research Network in Pediatric Infectious Diseases), Madrid, Spain cintamoraledaredecilla@gmail.com.
  • Domínguez-Rodríguez S; Instituto de Investigación 12 de Octubre (imas12), Fundación para la Investigación Biomédica del Hospital 12 de Octubre, Madrid, Spain.
  • Mesa JM; Instituto de Investigación 12 de Octubre (imas12), Fundación para la Investigación Biomédica del Hospital 12 de Octubre, Madrid, Spain.
  • García-Sánchez P; Paediatrics Department. Pediatrics Research Group, Hospital Universitario Infanta Sofia, San Sebastian de los Reyes, Madrid, Spain.
  • de la Serna M; Emergency Pediatric Department. Instituto Investigación Hospital La Paz (IDIPaz), Hospital Universitario La Paz, Madrid, Spain.
  • Alonso-Cadenas JA; Paediatrics Department. Pediatrics Research Group, Hospital Universitario Infanta Sofia, San Sebastian de los Reyes, Madrid, Spain.
  • Bermejo A; Emergency Pediatric Department. Instituto Investigación La Princesa, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
  • Sabrido G; Pediatric Department, Hospital Universitario de Móstoles, Móstoles, Madrid, Spain.
  • Martínez-Campos L; Pediatric Department, Hospital Universitario Rey Juan Carlos, Móstoles, Madrid, Spain.
  • González-Posada AF; Pediatric Infectious Diseases, Materno Infantil. Hospital Universitario Torrecárdenas, Almeria, Spain.
  • Illán-Ramos M; Emergency Pediatric Department, Hospital Universitario 12 de Octubre, Madrid, Spain.
  • Cobos-Carrascosa E; Pediatrics Department, Hospital Universitario Clínico San Carlos, Madrid, Spain.
  • Ballesteros Á; Instituto de Investigación 12 de Octubre (imas12), Fundación para la Investigación Biomédica del Hospital 12 de Octubre, Madrid, Spain.
  • Galán JC; Instituto de Investigación 12 de Octubre (imas12), Fundación para la Investigación Biomédica del Hospital 12 de Octubre, Madrid, Spain.
  • Llorente F; Microbiology Department, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Centro de Investigación Biomédica en Red en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
  • Aguilera-Alonso D; Centro de Investigacion en Sanidad Animal INIA-CSIC, Valdeolmos, Madrid, Spain.
  • Jiménez AB; Pediatric Infectious Diseases Unit. Department of Pediatrics, Hospital Universitario Gregorio Marañón. Unidad de Investigación Maternoinfantil Fundación Familia Alonso (UDIMIFFA), Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM). CIBER en Enfermedades Infecciosas (CIBERINFEC), Madrid, S
  • Catalán P; Pediatrics Department, Hospital Universitario Fundación Jiménez Díaz, Madrid, Spain.
  • Dahdouh E; Servicio de Microbiologia Clinica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • Navarro I; Clinical Microbiology and Parasitology Department, Hospital Universitario La Paz. IdiPAZ, Madrid, Spain.
  • Fernández-Garoz B; Paediatrics Department. Pediatrics Research Group, Hospital Universitario Infanta Sofia, San Sebastian de los Reyes, Madrid, Spain.
  • Mendoza P; Clinic Analysis Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
  • Pérez-Jorge C; Microbiology Department, Hospital Universitario de Móstoles, Mostoles, Madrid, Spain.
  • Cabezas-Fernández T; Microbiology Department, Hospital Universitario Rey Juan Carlos, Mostoles, Madrid, Spain.
  • Blázquez-Gamero D; Microbiology Department, Hospital Universitario Torrecárdenas, Almería, Spain.
  • Rivas G; Pediatric Infectious Diseases Unit. Department of Pediatrics, Hospital Universitario 12 de Octubre. Pediatric Research and Clinical Trials Unit (UPIC). RITIP (Translational Research Network in Pediatric Infectious Diseases), Madrid, Spain.
  • Gonzalez-Donapetry P; Instituto de Investigación 12 de Octubre (imas12), Fundación para la Investigación Biomédica del Hospital 12 de Octubre, Madrid, Spain.
  • Sáez E; Universidad Complutense, Madrid, Spain.
  • Fernández-Pinero J; Microbiology Department, Hospital Universitario 12 de Octubre, Madrid, Spain.
  • Lucas-Fernández J; Clinical Microbiology and Parasitology Department, Hospital Universitario La Paz. IdiPAZ, Madrid, Spain.
  • Pérez-Ramírez E; Microbiology Department, UR Salud. Hospital Universitario Infanta Sofía. Paediatrics Research Group, San Sebastian de los Reyes, Madrid, Spain.
  • Merino P; Centro de Investigacion en Sanidad Animal INIA-CSIC, Valdeolmos, Madrid, Spain.
  • Miragaya S; Microbiology Department, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Centro de Investigación Biomédica en Red en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
  • Lorente J; Centro de Investigacion en Sanidad Animal INIA-CSIC, Valdeolmos, Madrid, Spain.
  • Iglesias I; Microbiology Department, Hospital Universitario Clínico San Carlos, Madrid, Spain.
  • Tagarro A; Clinic Analysis Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
Arch Dis Child ; 107(11): 1051-1058, 2022 11.
Article in English | MEDLINE | ID: covidwho-1891766
ABSTRACT

OBJECTIVES:

To evaluate the performance of oral saliva swab (OSS) reverse transcription PCR (RT-PCR) compared with RT-PCR and antigen rapid diagnostic test (Ag-RDT) on nasopharyngeal swabs (NPS) for SARS-CoV-2 in children.

DESIGN:

Cross-sectional multicentre diagnostic study.

SETTING:

Study nested in a prospective, observational cohort (EPICO-AEP) performed between February and March 2021 including 10 hospitals in Spain. PATIENTS Children from 0 to 18 years with symptoms compatible with Covid-19 of ≤5 days of duration were included. Two NPS samples (Ag-RDT and RT-PCR) and one OSS sample for RT-PCR were collected. MAIN

OUTCOME:

Performance of Ag-RDT and RT-PCR on NPS and RT-PCR on OSS sample for SARS-CoV-2.

RESULTS:

1174 children were included, aged 3.8 years (IQR 1.7-9.0); 73/1174 (6.2%) patients tested positive by at least one of the techniques. Sensitivity and specificity of OSS RT-PCR were 72.1% (95% CI 59.7 to 81.9) and 99.6% (95% CI 99 to 99.9), respectively, versus 61.8% (95% CI 49.1 to 73) and 99.9% (95% CI 99.4 to 100) for the Ag-RDT. Kappa index was 0.79 (95% CI 0.72 to 0.88) for OSS RT-PCR and 0.74 (95% CI 0.65 to 0.84) for Ag-RDT versus NPS RT-PCR.

CONCLUSIONS:

RT-PCR on the OSS sample is an accurate option for SARS-CoV-2 testing in children. A less intrusive technique for younger patients, who usually are tested frequently, might increase the number of patients tested.
Subject(s)
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Child / Humans Language: English Journal: Arch Dis Child Year: 2022 Document Type: Article Affiliation country: Archdischild-2021-323712

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Child / Humans Language: English Journal: Arch Dis Child Year: 2022 Document Type: Article Affiliation country: Archdischild-2021-323712