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Significant role of host sialylated glycans in the infection and spread of severe acute respiratory syndrome coronavirus 2.
Saso, Wakana; Yamasaki, Masako; Nakakita, Shin-Ichi; Fukushi, Shuetsu; Tsuchimoto, Kana; Watanabe, Noriyuki; Sriwilaijaroen, Nongluk; Kanie, Osamu; Muramatsu, Masamichi; Takahashi, Yoshimasa; Matano, Tetsuro; Takeda, Makoto; Suzuki, Yasuo; Watashi, Koichi.
  • Saso W; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
  • Yamasaki M; The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
  • Nakakita SI; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
  • Fukushi S; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
  • Tsuchimoto K; Department of Applied Biological Sciences, Tokyo University of Science, Noda, Japan.
  • Watanabe N; Department of Functional Glycomics, Life Science Research Center, Kagawa University, Kagawa, Japan.
  • Sriwilaijaroen N; Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
  • Kanie O; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, Japan.
  • Muramatsu M; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
  • Takahashi Y; Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
  • Matano T; Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
  • Takeda M; Micro/Nano Technology Center and Department of Applied Biochemistry, Tokai University, Kanagawa, Japan.
  • Suzuki Y; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
  • Watashi K; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, Japan.
PLoS Pathog ; 18(6): e1010590, 2022 06.
Article in English | MEDLINE | ID: covidwho-1892333
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been transmitted across all over the world, in contrast to the limited epidemic of genetically- and virologically-related SARS-CoV. However, the molecular basis explaining the difference in the virological characteristics among SARS-CoV-2 and SARS-CoV has been poorly defined. Here we identified that host sialoglycans play a significant role in the efficient spread of SARS-CoV-2 infection, while this was not the case with SARS-CoV. SARS-CoV-2 infection was significantly inhibited by α2-6-linked sialic acid-containing compounds, but not by α2-3 analog, in VeroE6/TMPRSS2 cells. The α2-6-linked compound bound to SARS-CoV-2 spike S1 subunit to competitively inhibit SARS-CoV-2 attachment to cells. Enzymatic removal of cell surface sialic acids impaired the interaction between SARS-CoV-2 spike and angiotensin-converting enzyme 2 (ACE2), and suppressed the efficient spread of SARS-CoV-2 infection over time, in contrast to its least effect on SARS-CoV spread. Our study provides a novel molecular basis of SARS-CoV-2 infection which illustrates the distinctive characteristics from SARS-CoV.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: PLoS Pathog Year: 2022 Document Type: Article Affiliation country: Journal.ppat.1010590

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: PLoS Pathog Year: 2022 Document Type: Article Affiliation country: Journal.ppat.1010590