Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach

ABSTRACT

The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e¡06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (þ). This implies that the designed vaccine can prove effective in combating SARS-CoV-2. © 2022 University of Kerbala.