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Performance of the QIAprep& Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction.
Fenaux, Honorine; Limam, Lamia; Soutiere, Marie-Pierre; Veillet, Frédéric; Escuret, Vanessa; Roque-Afonso, Anne-Marie.
  • Fenaux H; Laboratoire de virologie, hôpital Paul Brousse, Assistance Publique, Hôpitaux de Paris, France.
  • Limam L; Laboratoire de virologie, hôpital Paul Brousse, Assistance Publique, Hôpitaux de Paris, France.
  • Soutiere MP; Laboratoire de virologie, hôpital Paul Brousse, Assistance Publique, Hôpitaux de Paris, France.
  • Veillet F; Laboratoire de virologie, hôpital Paul Brousse, Assistance Publique, Hôpitaux de Paris, France.
  • Escuret V; Hospices Civils de Lyon, Laboratoire associé au Centre National de Référence des virus des infections respiratoires, IAI, CBN, Lyon Cedex04, France; Univ Lyon, Université Claude Bernard Lyon 1, ENS Lyon, CIRI, Inserm U1111, CNRS UMR5308, Lyon, France.
  • Roque-Afonso AM; Laboratoire de virologie, hôpital Paul Brousse, Assistance Publique, Hôpitaux de Paris, France. Electronic address: anne-marie.roque@aphp.fr.
Diagn Microbiol Infect Dis ; 103(3): 115700, 2022 Jul.
Article in English | MEDLINE | ID: covidwho-1899670
ABSTRACT
We evaluated the performance of the QIAprep& Viral RNA UM Kit (Qiagen) for SARS-CoV-2 detection. It displayed specificity and sensitivity required for SARS-CoV-2 RNA detection from swab transport media without RNA extraction. This method identifies accurately patients at risk of transmission while saving time and cost of extraction.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article Affiliation country: J.diagmicrobio.2022.115700

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article Affiliation country: J.diagmicrobio.2022.115700