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Peptide Binder with High-Affinity for the SARS-CoV-2 Spike Receptor-Binding Domain.
Yu, Lanlan; Wang, Ruonan; Wen, Tao; Liu, Lei; Wang, Tao; Liu, Shuli; Xu, Haiyan; Wang, Chenxuan.
  • Yu L; State Key Laboratory of Medical Molecular Biology, Haihe Laboratory of Cell Ecosystem, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
  • Wang R; State Key Laboratory of Medical Molecular Biology, Haihe Laboratory of Cell Ecosystem, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
  • Wen T; Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
  • Liu L; Department of Chemistry, University of Chicago, Chicago, Illinois 60637, United States.
  • Wang T; Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
  • Liu S; Department of Clinical Laboratory, Peking University Civil Aviation School of Clinical Medicine, Beijing 100123, China.
  • Xu H; Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
  • Wang C; State Key Laboratory of Medical Molecular Biology, Haihe Laboratory of Cell Ecosystem, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
ACS Appl Mater Interfaces ; 14(25): 28527-28536, 2022 Jun 29.
Article in English | MEDLINE | ID: covidwho-1900420
ABSTRACT
Rapid antigen detection tests are urgently needed for the early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The discovery of a binder with high affinity and selectivity for the biomarkers presented by SARS-CoV-2 is crucial to the development of the rapid antigen detection method. We utilized the surface biopanning to identify a peptide binder R1 from a phage-displayed peptide library consisting of 109 independent phage recombinants. The R1 peptide exhibited high-affinity for specific binding with the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein with a dissociation constant KD of (7.5 ± 1.9) × 10-10 M, which maintained high binding affinity with the RBD derived from Gamma, Lambda, Delta, and Omicron variants. The composition and sequence dependence of binding characteristics in R1-RBD interactions was revealed by the binding affinity fluctuations between RBD and the scrambled sequences or single-site mutants of R1. The R1-functionalized gold nanoparticles possessed concentration-dependent response to RBD and selectivity over bovine serum albumin and human serum albumin. The peptide binder R1 shows the potential to be used for constructing a rapid detection method for the early-stage diagnostics for SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Metal Nanoparticles / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Topics: Variants Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article Affiliation country: Acsami.2c03707

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Metal Nanoparticles / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Topics: Variants Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article Affiliation country: Acsami.2c03707