Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration.
Lab Chip
; 22(14): 2695-2706, 2022 07 12.
Article
in English
| MEDLINE | ID: covidwho-1900680
ABSTRACT
Heterogeneous immunoassays (HI) are an invaluable tool for biomarker detection and remain an ideal candidate for microfluidic point-of-care diagnostics. However, automating and controlling sustained fluid flow from benchtop to microfluidics for the HI reaction during the extended sample incubation step, remains difficult to implement; this leads to challenges for assay integration and assay result interpretation. To address these issues, we investigated the liquid reciprocation process on a microfluidic centrifugal disc (CD) to generate continuous, bidirectional fluid flow using only a rotating motor. Large volumetric flow rates (µL s-1) through the HI reaction chamber were sustained for extended durations (up to 1 h). The CD liquid reciprocation operating behavior was characterized experimentally and simulated to determine fluid flow shear rates through our HI reaction chamber. We demonstrated the continuous CD liquid reciprocation for target molecule incubation for a microarray HI and that higher fluid shear rates negatively influenced our fluorescence intensity. We highlight the importance of proper fluid flow considerations when integrating HIs with microfluidics.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Microfluidic Analytical Techniques
/
COVID-19
Limits:
Humans
Language:
English
Journal:
Lab Chip
Journal subject:
Biotechnology
/
Chemistry
Year:
2022
Document Type:
Article
Affiliation country:
D2lc00213b
Similar
MEDLINE
...
LILACS
LIS