Two Biosensors for the Determination of Interleukin-6 in Blood Plasma by Array SPRi.
Biosensors (Basel)
; 12(6)2022 Jun 14.
Article
in English
| MEDLINE | ID: covidwho-1903262
ABSTRACT
Interleukin-6 (IL-6) is a biomarker of inflammation, the advanced stage of COVID-19, and several cancers, including ovarian cancer. Two biosensors for the determination of IL-6 in blood plasma by array SPRi have been developed. One of these biosensors consists of the mouse monoclonal anti-IL-6 antibody as the receptor immobilized via the cysteamine linker. The second contains galiellalactone as the receptor, being an inhibitor specific for IL-6, immobilized via octadecanethiol (ODM) as the linker. Both biosensors are specific for IL-6. The biosensor with the antibody as the receptor gives a linear analytical response between 3 (LOQ) and 20 pg mL-1 and has a precision between 8% and 9.8% and recovery between 97% and 107%, depending on the IL-6 concentration. The biosensor with galiellalactone as the receptor gives a linear analytical response between 1.1 (LOQ) and 20 pg mL-1, and has a precision between 3.5% and 9.3% and recovery between 101% and 105%, depending on IL-6 concentration. Both biosensors were validated. Changes in IL-6 concentration in blood plasma before and after resection of ovarian tumor and endometrial cyst, as determined by the two developed biosensors, are given as an example of a real clinical application.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Ovarian Neoplasms
/
Biosensing Techniques
/
COVID-19
Type of study:
Experimental Studies
/
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
English
Year:
2022
Document Type:
Article
Affiliation country:
Bios12060412
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