Your browser doesn't support javascript.
Evaluation of serological assays for SARS-CoV-2 antibody testing from dried blood spots collected from cohorts with prior SARS-CoV-2 infection.
Catlett, Beth; Starr, Mitchell; Machalek, Dorothy A; Danwilai, Thidarat; Palmer, Michael; Kelly, Andrew; Kaldor, John; Dore, Gregory J; Darley, David; Matthews, Gail; Cunningham, Philip H.
  • Catlett B; NSW State Reference Laboratory for HIV, St Vincent's Centre for Applied Medical Research, Darlinghurst, Sydney, New South Wales, Australia.
  • Starr M; The Kirby Institute, UNSW Sydney, Sydney, New South Wales, Australia.
  • Machalek DA; St Vincent's Hospital, Sydney, New South Wales, Australia.
  • Danwilai T; NSW State Reference Laboratory for HIV, St Vincent's Centre for Applied Medical Research, Darlinghurst, Sydney, New South Wales, Australia.
  • Palmer M; St Vincent's Hospital, Sydney, New South Wales, Australia.
  • Kelly A; The Kirby Institute, UNSW Sydney, Sydney, New South Wales, Australia.
  • Kaldor J; Centre for Women's Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
  • Dore GJ; NSW State Reference Laboratory for HIV, St Vincent's Centre for Applied Medical Research, Darlinghurst, Sydney, New South Wales, Australia.
  • Darley D; St Vincent's Hospital, Sydney, New South Wales, Australia.
  • Matthews G; NSW State Reference Laboratory for HIV, St Vincent's Centre for Applied Medical Research, Darlinghurst, Sydney, New South Wales, Australia.
  • Cunningham PH; St Vincent's Hospital, Sydney, New South Wales, Australia.
J Clin Virol Plus ; 2(3): 100093, 2022 Aug.
Article in English | MEDLINE | ID: covidwho-2254022
ABSTRACT

Background:

Dried blood spot (DBS) specimens are a useful serosurveillance tool particularly in hard-to-reach populations but their application for detecting SARS-CoV-2 infection is poorly characterised.

Objectives:

To compare detection of naturally acquired SARS-CoV-2 antibodies in paired DBS and serum specimens using commercially available serological immunoassays. Study

Design:

Specimens were collected through St Vincent's Hospital observational post COVID-19 cohort study (ADAPT). Laboratory spotted DBS from venepuncture were initially tested on seven assays, a DBS validation completed on three with clinically collected fingerstick DBSs tested on one.

Results:

Sensitivity for Euroimmun nucleocapsid (NCP) IgG ELISA from laboratory spotted DBS (n=145), Euroimmun spike, IgG ELISA from laboratory spotted DBS (n=161), and Binding Site total antibody ELISA from clinically collected fingerstick DBS (n=391) was 100% (95% CI 95.8-100%), 100% (95% CI 95.8-100%) and 92.9% (95% CI 89.5-95.5%), respectively. Specificity was 66.2% (95% CI 53.6-77.0%), 96% (95% CI 88.7-99.1%) and 98.8% (95% CI 93.3-99.9%), respectively. All three assays' results displayed a strong positive correlation between DBS compared to paired serum.

Conclusions:

The Binding Site™ spike total antibody and Euroimmun™ spike IgG ELISAs provided good analytical performance, demonstrating that DBS specimens could facilitate specimen collection in the epidemiological surveillance of SARS-CoV-2 infection. This is highly applicable in populations and settings where venepuncture is problematic (including community based regional/remote settings, nursing homes, prisons, and schools).
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Long Covid Language: English Journal: J Clin Virol Plus Year: 2022 Document Type: Article Affiliation country: J.jcvp.2022.100093

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Long Covid Language: English Journal: J Clin Virol Plus Year: 2022 Document Type: Article Affiliation country: J.jcvp.2022.100093