Evaluation of serological assays for SARS-CoV-2 antibody testing from dried blood spots collected from cohorts with prior SARS-CoV-2 infection.
J Clin Virol Plus
; 2(3): 100093, 2022 Aug.
Article
in English
| MEDLINE | ID: covidwho-2254022
ABSTRACT
Background:
Dried blood spot (DBS) specimens are a useful serosurveillance tool particularly in hard-to-reach populations but their application for detecting SARS-CoV-2 infection is poorly characterised.Objectives:
To compare detection of naturally acquired SARS-CoV-2 antibodies in paired DBS and serum specimens using commercially available serological immunoassays. StudyDesign:
Specimens were collected through St Vincent's Hospital observational post COVID-19 cohort study (ADAPT). Laboratory spotted DBS from venepuncture were initially tested on seven assays, a DBS validation completed on three with clinically collected fingerstick DBSs tested on one.Results:
Sensitivity for Euroimmun nucleocapsid (NCP) IgG ELISA from laboratory spotted DBS (n=145), Euroimmun spike, IgG ELISA from laboratory spotted DBS (n=161), and Binding Site total antibody ELISA from clinically collected fingerstick DBS (n=391) was 100% (95% CI 95.8-100%), 100% (95% CI 95.8-100%) and 92.9% (95% CI 89.5-95.5%), respectively. Specificity was 66.2% (95% CI 53.6-77.0%), 96% (95% CI 88.7-99.1%) and 98.8% (95% CI 93.3-99.9%), respectively. All three assays' results displayed a strong positive correlation between DBS compared to paired serum.Conclusions:
The Binding Site™ spike total antibody and Euroimmun™ spike IgG ELISAs provided good analytical performance, demonstrating that DBS specimens could facilitate specimen collection in the epidemiological surveillance of SARS-CoV-2 infection. This is highly applicable in populations and settings where venepuncture is problematic (including community based regional/remote settings, nursing homes, prisons, and schools).
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Type of study:
Cohort study
/
Diagnostic study
/
Experimental Studies
/
Observational study
/
Prognostic study
Topics:
Long Covid
Language:
English
Journal:
J Clin Virol Plus
Year:
2022
Document Type:
Article
Affiliation country:
J.jcvp.2022.100093
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