Your browser doesn't support javascript.
Conjunctival epithelial cells resist productive SARS-CoV-2 infection.
Jackson, Robert M; Hatton, Catherine F; Spegarova, Jarmila Stremenova; Georgiou, Maria; Collin, Joseph; Stephenson, Emily; Verdon, Bernard; Haq, Iram J; Hussain, Rafiqul; Coxhead, Jonathan M; Mudhar, Hardeep-Singh; Wagner, Bart; Hasoon, Megan; Davey, Tracey; Rooney, Paul; Khan, C M Anjam; Ward, Chris; Brodlie, Malcolm; Haniffa, Muzlifah; Hambleton, Sophie; Armstrong, Lyle; Figueiredo, Francisco; Queen, Rachel; Duncan, Christopher J A; Lako, Majlinda.
  • Jackson RM; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Hatton CF; Translational and Clinical Research Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Spegarova JS; Translational and Clinical Research Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Georgiou M; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Collin J; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Stephenson E; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Verdon B; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Haq IJ; Translational and Clinical Research Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Hussain R; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Coxhead JM; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Mudhar HS; National Specialist Ophthalmic Pathology Service (NSOPS) Department of Histopathology, E-Floor, Royal Hallamshire Hospital, Sheffield, UK.
  • Wagner B; Electron Microscopy Unit, Royal Hallamshire Hospital, Sheffield, UK.
  • Hasoon M; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Davey T; Electron Microscopy Unit, Royal Hallamshire Hospital, Sheffield, UK.
  • Rooney P; NHS Blood and Transplant Tissue and Eye Services, Liverpool, UK.
  • Khan CMA; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Ward C; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Brodlie M; Translational and Clinical Research Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Haniffa M; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK; Department of Dermatology and NIHR Newcastle Biomedical Research Centre, Newcastle Hospitals NHS Foundation Trust, Newcastle Upon Tyne, UK; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
  • Hambleton S; Translational and Clinical Research Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Armstrong L; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.
  • Figueiredo F; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK; Department of Ophthalmology, Royal Victoria Infirmary and Newcastle University, Newcastle, UK.
  • Queen R; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK. Electronic address: rachel.queen@ncl.ac.uk.
  • Duncan CJA; Translational and Clinical Research Institute, Newcastle University, Newcastle Upon Tyne, UK. Electronic address: christopher.duncan@ncl.ac.uk.
  • Lako M; Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK. Electronic address: majlinda.lako@ncl.ac.uk.
Stem Cell Reports ; 17(7): 1699-1713, 2022 07 12.
Article in English | MEDLINE | ID: covidwho-1907809
ABSTRACT
Conjunctival epithelial cells, which express viral-entry receptors angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine type 2 (TMPRSS2), constitute the largest exposed epithelium of the ocular surface tissue and may represent a relevant viral-entry route. To address this question, we generated an organotypic air-liquid-interface model of conjunctival epithelium, composed of basal, suprabasal, and superficial epithelial cells, and fibroblasts, which could be maintained successfully up to day 75 of differentiation. Using single-cell RNA sequencing (RNA-seq), with complementary imaging and virological assays, we observed that while all conjunctival cell types were permissive to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome expression, a productive infection did not ensue. The early innate immune response to SARS-CoV-2 infection in conjunctival cells was characterised by a robust autocrine and paracrine NF-κB activity, without activation of antiviral interferon signalling. Collectively, these data enrich our understanding of SARS-CoV-2 infection at the human ocular surface, with potential implications for the design of preventive strategies and conjunctival transplantation.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Limits: Humans Language: English Journal: Stem Cell Reports Year: 2022 Document Type: Article Affiliation country: J.stemcr.2022.05.017

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Limits: Humans Language: English Journal: Stem Cell Reports Year: 2022 Document Type: Article Affiliation country: J.stemcr.2022.05.017