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Self-collected nasopharyngeal swab and molecular test using pool testing as strategies to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, Brazil, 2021. / Autocoleta de swab nasofaríngeo e teste molecular em pool testing como estratégias para detecção de coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2): viabilidade em estudantes de medicina da Universidade Federal de Minas Gerais, 2021.
Guimarães, Nathalia Sernizon; Costa, Murilo Soares; Machado, Elaine Leandro; Sato, Hugo Itaru; Amaral, Eduarda de Carvalho Maia E; Arivabene, Rafaela Galvão; Lourenço, Karine Lima; Tupinambás, Unaí; Fonseca, Flávio Guimarães da; Takahashi, Ricardo Hiroshi Caldeira; Teixeira, Santuza Maria Ribeiro; Alves, Claudia Regina Lindgren.
  • Guimarães NS; Universidade Federal de Minas Gerais, Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Belo Horizonte, MG, Brasil.
  • Costa MS; Universidade Federal de Minas Gerais, Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Belo Horizonte, MG, Brasil.
  • Machado EL; Universidade Federal de Minas Gerais, Departamento de Medicina Preventiva e Social, Belo Horizonte, MG, Brasil.
  • Sato HI; Universidade Federal de Minas Gerais, Centro de Tecnologia de Vacinas, Belo Horizonte, MG, Brasil.
  • Amaral ECME; Universidade Federal de Minas Gerais, Faculdade de Medicina, Belo Horizonte, MG, Brasil.
  • Arivabene RG; Universidade Federal de Minas Gerais, Faculdade de Medicina, Belo Horizonte, MG, Brasil.
  • Lourenço KL; Universidade Federal de Minas Gerais, Centro de Tecnologia de Vacinas, Belo Horizonte, MG, Brasil.
  • Tupinambás U; Universidade Federal de Minas Gerais, Departamento de Clínica Médica, Belo Horizonte, MG, Brasil.
  • Fonseca FGD; Universidade Federal de Minas Gerais, Centro de Tecnologia de Vacinas, Belo Horizonte, MG, Brasil.
  • Takahashi RHC; Universidade Federal de Minas Gerais, Departamento de Matemática, Belo Horizonte, MG, Brasil.
  • Teixeira SMR; Universidade Federal de Minas Gerais, Centro de Tecnologia de Vacinas, Belo Horizonte, MG, Brasil.
  • Alves CRL; Universidade Federal de Minas Gerais, Departamento de Pediatria, Belo Horizonte, MG, Brasil.
Epidemiol Serv Saude ; 31(1): e2021409, 2022.
Article in English, Portuguese | MEDLINE | ID: covidwho-1910771
ABSTRACT

OBJECTIVE:

To show the feasibility of the combined use of self-collected nasopharyngeal swab and pool testing to detect SARS-CoV-2 in epidemiological surveys.

METHODS:

This experience included a sample of 154 students at the Universidade Federal de Minas Gerais, who performed self-collected nasopharyngeal swab in individual cabins and without supervision. The molecular test was performed using the pool testing technique.

RESULTS:

It took each person 5 minutes to collect the sample. An analysis was performed to detect endogenous RNA in 40 samples. The results showed that there were no failures resulting from self-collection. None of the pools detected the presence of viral RNA. The cost of molecular testing (RT-PCR), by pool testing, with samples obtained by self-collection was about ten times lower than the usual methods.

CONCLUSION:

The strategies that were investigated proved to be economically feasible and valid for the research on SARS-CoV-2 in epidemiological surveys.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Students, Medical / COVID-19 Type of study: Diagnostic study / Observational study Limits: Humans Country/Region as subject: South America / Brazil Language: English / Portuguese Journal: Epidemiol Serv Saude Year: 2022 Document Type: Article Affiliation country: S1679-49742022000100002

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Students, Medical / COVID-19 Type of study: Diagnostic study / Observational study Limits: Humans Country/Region as subject: South America / Brazil Language: English / Portuguese Journal: Epidemiol Serv Saude Year: 2022 Document Type: Article Affiliation country: S1679-49742022000100002