Your browser doesn't support javascript.
Development and Validation of Indirect Enzyme-Linked Immunosorbent Assays for Detecting Antibodies to SARS-CoV-2 in Cattle, Swine, and Chicken.
Gontu, Abhinay; Marlin, Erika A; Ramasamy, Santhamani; Neerukonda, Sabarinath; Anil, Gayatri; Morgan, Jasmine; Quraishi, Meysoon; Chen, Chen; Boorla, Veda Sheersh; Nissly, Ruth H; Jakka, Padmaja; Chothe, Shubhada K; Ravichandran, Abirami; Kodali, Nishitha; Amirthalingam, Saranya; LaBella, Lindsey; Kelly, Kathleen; Natesan, Pazhanivel; Minns, Allen M; Rossi, Randall M; Werner, Jacob R; Hovingh, Ernest; Lindner, Scott E; Tewari, Deepanker; Kapur, Vivek; Vandegrift, Kurt J; Maranas, Costas D; Surendran Nair, Meera; Kuchipudi, Suresh V.
  • Gontu A; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Marlin EA; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Ramasamy S; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Neerukonda S; Clinical & Diagnostic Assay Development Group, Pfizer, Pearl River, NY 10965, USA.
  • Anil G; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Morgan J; U.S. Department of Health and Human Services, Silver Spring, MD 20993, USA.
  • Quraishi M; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Chen C; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Boorla VS; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Nissly RH; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
  • Jakka P; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
  • Chothe SK; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Ravichandran A; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Kodali N; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Amirthalingam S; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • LaBella L; Department of Integrative and Biomedical Physiology, The Pennsylvania State University, University Park, PA 16802, USA.
  • Kelly K; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Natesan P; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Minns AM; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Rossi RM; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Werner JR; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Hovingh E; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Lindner SE; Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
  • Tewari D; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Kapur V; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
  • Vandegrift KJ; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Maranas CD; Department of Animal Science, The Pennsylvania State University, University Park, PA 16802, USA.
  • Surendran Nair M; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Kuchipudi SV; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
Viruses ; 14(7)2022 06 22.
Article in English | MEDLINE | ID: covidwho-1911645
ABSTRACT
Multiple domestic and wild animal species are susceptible to SARS-CoV-2 infection. Cattle and swine are susceptible to experimental SARS-CoV-2 infection. The unchecked transmission of SARS-CoV-2 in animal hosts could lead to virus adaptation and the emergence of novel variants. In addition, the spillover and subsequent adaptation of SARS-CoV-2 in livestock could significantly impact food security as well as animal and public health. Therefore, it is essential to monitor livestock species for SARS-CoV-2 spillover. We developed and optimized species-specific indirect ELISAs (iELISAs) to detect anti-SARS-CoV-2 antibodies in cattle, swine, and chickens using the spike protein receptor-binding domain (RBD) antigen. Serum samples collected prior to the COVID-19 pandemic were used to determine the cut-off threshold. RBD hyperimmunized sera from cattle (n = 3), swine (n = 6), and chicken (n = 3) were used as the positive controls. The iELISAs were evaluated compared to a live virus neutralization test using cattle (n = 150), swine (n = 150), and chicken (n = 150) serum samples collected during the COVID-19 pandemic. The iELISAs for cattle, swine, and chicken were found to have 100% sensitivity and specificity. These tools facilitate the surveillance that is necessary to quickly identify spillovers into the three most important agricultural species worldwide.
Subject(s)
Keywords
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Variants Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: V14071358

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Variants Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: V14071358