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Intranasal administration of a live-attenuated recombinant newcastle disease virus expressing the SARS-CoV-2 Spike protein induces high neutralizing antibody levels and protects from experimental challenge infection in hamsters.
de Swart, Rik L; de Leeuw, Olav S; Oreshkova, Nadia; Gerhards, Nora M; Albulescu, Irina C; Vreman, Sandra; Gonzales, José L; Maas, Riks; van Kuppeveld, Frank J M; Soema, Peter; Bosch, Berend-Jan; Peeters, Ben P H.
  • de Swart RL; Wageningen Bioveterinary Research, Lelystad, Netherlands. Electronic address: rik.deswart@wur.nl.
  • de Leeuw OS; Wageningen Bioveterinary Research, Lelystad, Netherlands.
  • Oreshkova N; Wageningen Bioveterinary Research, Lelystad, Netherlands.
  • Gerhards NM; Wageningen Bioveterinary Research, Lelystad, Netherlands.
  • Albulescu IC; Department Biomolecular Health Sciences, Division Infectious Diseases & Immunology - Virology section, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.
  • Vreman S; Wageningen Bioveterinary Research, Lelystad, Netherlands.
  • Gonzales JL; Wageningen Bioveterinary Research, Lelystad, Netherlands.
  • Maas R; Wageningen Bioveterinary Research, Lelystad, Netherlands.
  • van Kuppeveld FJM; Department Biomolecular Health Sciences, Division Infectious Diseases & Immunology - Virology section, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.
  • Soema P; Intravacc, Bilthoven, Netherlands.
  • Bosch BJ; Department Biomolecular Health Sciences, Division Infectious Diseases & Immunology - Virology section, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.
  • Peeters BPH; Wageningen Bioveterinary Research, Lelystad, Netherlands.
Vaccine ; 40(33): 4676-4681, 2022 08 05.
Article in English | MEDLINE | ID: covidwho-1915072
ABSTRACT
The emergence of SARS-CoV-2 in December 2019 resulted in the COVID-19 pandemic. Recurring disease outbreaks repeatedly overloaded the public health sector and severely affected the global economy. We developed a candidate COVID-19 vaccine based on a recombinant Newcastle disease virus (NDV) vaccine vector, encoding a pre-fusion stabilized full-length Spike protein obtained from the original SARS-CoV-2 Wuhan isolate. Vaccination of hamsters by intra-muscular injection or intra-nasal instillation induced high neutralizing antibody responses. Intranasal challenge infection with SARS-CoV-2 strain Lelystad demonstrated that both vaccination routes provided partial protection in the upper respiratory tract, and almost complete protection in the lower respiratory tract, as measured by suppressed viral loads and absence of histological lung lesions. Activity wheel measurements demonstrated that animals vaccinated by intranasal inoculation rapidly recovered to normal activity. NDV constructs encoding the spike of SARS-CoV-2 may be attractive candidates for development of intra-nasal COVID-19 booster vaccines.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Topics: Vaccines Limits: Animals / Humans Language: English Journal: Vaccine Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Topics: Vaccines Limits: Animals / Humans Language: English Journal: Vaccine Year: 2022 Document Type: Article