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Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies.
Kim, Sun Jin; Yao, Zhong; Marsh, Morgan C; Eckert, Debra M; Kay, Michael S; Lyakisheva, Anna; Pasic, Maria; Bansal, Aiyush; Birnboim, Chaim; Jha, Prabhat; Galipeau, Yannick; Langlois, Marc-André; Delgado, Julio C; Elgort, Marc G; Campbell, Robert A; Middleton, Elizabeth A; Stagljar, Igor; Owen, Shawn C.
  • Kim SJ; Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA.
  • Yao Z; Donnelly Centre, University of Toronto, Ontario, Canada.
  • Marsh MC; Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USA.
  • Eckert DM; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA.
  • Kay MS; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA.
  • Lyakisheva A; Donnelly Centre, University of Toronto, Ontario, Canada.
  • Pasic M; Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada.
  • Bansal A; Department of Laboratory Medicine, St. Joseph's Health Centre, Toronto, Ontario, Canada.
  • Birnboim C; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada.
  • Jha P; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada.
  • Galipeau Y; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada.
  • Langlois MA; Centre for Global Health Research, Unity Health Toronto, University of Toronto, Toronto, Canada.
  • Delgado JC; Department of Biochemistry, Microbiology & Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada.
  • Elgort MG; Department of Biochemistry, Microbiology & Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada.
  • Campbell RA; University of Ottawa Centre for Infection, Immunity and Inflammation (CI3), Ottawa, Canada.
  • Middleton EA; ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.
  • Stagljar I; Department of Pathology, University of Utah, Salt Lake City, UT, USA.
  • Owen SC; ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.
Nat Commun ; 13(1): 3716, 2022 07 01.
Article in English | MEDLINE | ID: covidwho-1984382
ABSTRACT
The COVID-19 pandemic triggered the development of numerous diagnostic tools to monitor infection and to determine immune response. Although assays to measure binding antibodies against SARS-CoV-2 are widely available, more specific tests measuring neutralization activities of antibodies are immediately needed to quantify the extent and duration of protection that results from infection or vaccination. We previously developed a 'Serological Assay based on a Tri-part split-NanoLuc® (SATiN)' to detect antibodies that bind to the spike (S) protein of SARS-CoV-2. Here, we expand on our previous work and describe a reconfigured version of the SATiN assay, called Neutralization SATiN (Neu-SATiN), which measures neutralization activity of antibodies directly from convalescent or vaccinated sera. The results obtained with our assay and other neutralization assays are comparable but with significantly shorter preparation and run time for Neu-SATiN. As the assay is modular, we further demonstrate that Neu-SATiN enables rapid assessment of the effectiveness of vaccines and level of protection against existing SARS-CoV-2 variants of concern and can therefore be readily adapted for emerging variants.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Vaccines / Variants Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2022 Document Type: Article Affiliation country: S41467-022-31300-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Vaccines / Variants Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2022 Document Type: Article Affiliation country: S41467-022-31300-9