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Electrochemical Determination of Interaction between SARS-CoV-2 Spike Protein and Specific Antibodies.
Drobysh, Maryia; Liustrovaite, Viktorija; Baradoke, Ausra; Rucinskiene, Alma; Ramanaviciene, Almira; Ratautaite, Vilma; Viter, Roman; Chen, Chien-Fu; Plikusiene, Ieva; Samukaite-Bubniene, Urte; Slibinskas, Rimantas; Ciplys, Evaldas; Simanavicius, Martynas; Zvirbliene, Aurelija; Kucinskaite-Kodze, Indre; Ramanavicius, Arunas.
  • Drobysh M; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Liustrovaite V; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania.
  • Baradoke A; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Rucinskiene A; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania.
  • Ramanaviciene A; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Ratautaite V; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania.
  • Viter R; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Chen CF; State Research Institute Center of Innovative Medicine, LT-08406 Vilnius, Lithuania.
  • Plikusiene I; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Samukaite-Bubniene U; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania.
  • Slibinskas R; Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1004 Riga, Latvia.
  • Ciplys E; Center for Collective Use of Research Equipment, Sumy State University, 40000 Sumy, Ukraine.
  • Simanavicius M; Institute of Applied Mechanics, National Taiwan University, Taipei City 106, Taiwan.
  • Zvirbliene A; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Kucinskaite-Kodze I; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Ramanavicius A; Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania.
Int J Mol Sci ; 23(12)2022 Jun 17.
Article in English | MEDLINE | ID: covidwho-1917510
ABSTRACT
The serologic diagnosis of coronavirus disease 2019 (COVID-19) and the evaluation of vaccination effectiveness are identified by the presence of antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we present the electrochemical-based biosensing technique for the detection of antibodies specific to the SARS-CoV-2 proteins. Recombinant SARS-CoV-2 spike proteins (rSpike) were immobilised on the surface of a gold electrode modified by a self-assembled monolayer (SAM). This modified electrode was used as a sensitive element for the detection of polyclonal mouse antibodies against the rSpike (anti-rSpike). Electrochemical impedance spectroscopy (EIS) was used to observe the formation of immunocomplexes while cyclic voltammetry (CV) was used for additional analysis of the surface modifications. It was revealed that the impedimetric method and the elaborate experimental conditions are appropriate for the further development of electrochemical biosensors for the serological diagnosis of COVID-19 and/or the confirmation of successful vaccination against SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23126768

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23126768