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Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen.
Shahdeo, Deepshikha; Chauhan, Neha; Majumdar, Aniket; Ghosh, Arindam; Gandhi, Sonu.
  • Shahdeo D; DBT-National Institute of Animal Biotechnology (DBT-NIAB), Hyderabad 500032, Telangana, India.
  • Chauhan N; Department of Physics, Indian Institute of Science (IISc), Bangalore 560012, India.
  • Majumdar A; The Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore 560012, India.
  • Ghosh A; Department of Physics, Indian Institute of Science (IISc), Bangalore 560012, India.
  • Gandhi S; Department of Physics, Indian Institute of Science (IISc), Bangalore 560012, India.
ACS Appl Bio Mater ; 5(7): 3563-3572, 2022 07 18.
Article in English | MEDLINE | ID: covidwho-1921549
ABSTRACT
Coronavirus disease (COVID-19) is an infectious disease that has posed a global health challenge caused by the SARS-CoV-2 virus. Early management and diagnosis of SARS-CoV-2 are crucial for the timely treatment, traceability, and reduction of viral spread. We have developed a rapid method using a Graphene-based Field-Effect Transistor (Gr-FET) for the ultrasensitive detection of SARS-CoV-2 Spike S1 antigen (S1-Ag). The in-house developed antispike S1 antibody (S1-Ab) was covalently immobilized on the surface of a carboxy functionalized graphene channel using carbodiimide chemistry. Ultraviolet-visible spectroscopy, Fourier-Transform Infrared Spectroscopy, X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Optical Microscopy, Raman Spectroscopy, Scanning Electron Microscopy (SEM), Enzyme-Linked Immunosorbent Assays (ELISA), and device stability studies were conducted to characterize the bioconjugation and fabrication process of Gr-FET. In addition, the electrical response of the device was evaluated by monitoring the change in resistance caused by Ag-Ab interaction in real time. For S1-Ag, our Gr-FET devices were tested in the range of 1 fM to 1 µM with a limit of detection of 10 fM in the standard buffer. The fabricated devices are highly sensitive, specific, and capable of detecting low levels of S1-Ag.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies Limits: Humans Language: English Journal: ACS Appl Bio Mater Year: 2022 Document Type: Article Affiliation country: Acsabm.2c00503

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies Limits: Humans Language: English Journal: ACS Appl Bio Mater Year: 2022 Document Type: Article Affiliation country: Acsabm.2c00503