Improvement of the Detection Method for the Serum Titer of the Inactivated SARS-CoV-2 Vaccine

ABSTRACT

A mixed antigen coating method was designed to optimize the method for detecting the titer of the immune serum to inactivated SARS-CoV-2 vaccine, which based on the traditional single antigen coating.The authors determined the optimal coating concentration of the single antigen first by the checkerboard method, then combined two kinds of antigen and obtained coating concentration of the mixed antigen.The best combination of mixed coating antigen contains the whole-coronavirus antigen with total protein concentration of 25 ng/well and the recombinant new coro-navirus S1 protein antigen with protein concentration of 50 ng/well.This method can be used to detect all antibody-specific titers in serum effectively, especially serum containing high-level S protein-specific antibodies.