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Association of perturbation of oral bacterial with incident of Alzheimer's disease: A pilot study.
Taati Moghadam, Majid; Amirmozafari, Nour; Mojtahedi, Ali; Bakhshayesh, Babak; Shariati, Aref; Masjedian Jazi, Faramarz.
  • Taati Moghadam M; Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.
  • Amirmozafari N; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Mojtahedi A; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Bakhshayesh B; Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
  • Shariati A; Department of Neurology, Poursina Hospital, Guilan University of Medical Sciences, Rasht, Iran.
  • Masjedian Jazi F; Molecular and medicine research center, Khomein University of Medical Sciences, Khomein, Iran.
J Clin Lab Anal ; 36(7): e24483, 2022 Jul.
Article in English | MEDLINE | ID: covidwho-1929823
ABSTRACT

OBJECTIVE:

This case-control study was designed to compare the composition of the predominant oral bacterial microbiome in Alzheimer's disease (AD) and control group. SUBJECT A total of 30 adult participants (15 AD and 15 healthy individuals) were entered in this study. The composition of oral bacterial microbiome was examined by quantitative real-time polymerase chain reaction (qPCR) using bacterial 16S rDNA gene. The levels of systemic inflammatory cytokines in both groups were assessed using enzyme-linked immunosorbent assays (ELISA).

RESULTS:

The loads of Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia were significantly more abundant in the AD compared to the control group (p < 0.05). Although Aggregatibacter actinomycetemcomitans and Streptococcus mutans were relatively frequent in the AD group, no significance difference was observed in their copy number between two groups. Although the concentrations of IL-1, IL-6, and TNF-α were higher in the AD group, there was a significant difference in their levels between the two groups (p < 0.05). Finally, there was a significant relationship between increased number of pathogenic bacteria in oral microbiome and higher concentration of cytokines in patient's blood.

CONCLUSION:

Our knowledge of oral microbiome and its exact association with AD is rather limited; our study showed a significant association between changes in oral microbiome bacteria, increased inflammatory cytokines, and AD.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Alzheimer Disease / Microbiota / Mouth Type of study: Experimental Studies / Observational study / Randomized controlled trials Limits: Adult / Humans Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Jcla.24483

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Alzheimer Disease / Microbiota / Mouth Type of study: Experimental Studies / Observational study / Randomized controlled trials Limits: Adult / Humans Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Jcla.24483