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An integrated analysis and comparison of serum, saliva and sebum for COVID-19 metabolomics.
Spick, Matt; Lewis, Holly-May; Frampas, Cecile F; Longman, Katie; Costa, Catia; Stewart, Alexander; Dunn-Walters, Deborah; Greener, Danni; Evetts, George; Wilde, Michael J; Sinclair, Eleanor; Barran, Perdita E; Skene, Debra J; Bailey, Melanie J.
  • Spick M; Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Lewis HM; Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Frampas CF; Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Longman K; Faculty of Health and Medical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Costa C; Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Stewart A; Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Dunn-Walters D; Surrey Ion Beam Centre, University of Surrey, Guildford, GU2 7XH, UK.
  • Greener D; Faculty of Health and Medical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Evetts G; Faculty of Health and Medical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
  • Wilde MJ; Frimley Park Hospital, Frimley Health NHS Trust, Frimley, GU16 7UJ, UK.
  • Sinclair E; Frimley Park Hospital, Frimley Health NHS Trust, Frimley, GU16 7UJ, UK.
  • Barran PE; School of Geography, Earth and Environmental Sciences, University of Plymouth, Plymouth, PL4 8AA, UK.
  • Skene DJ; Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK.
  • Bailey MJ; Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK.
Sci Rep ; 12(1): 11867, 2022 07 13.
Article in English | MEDLINE | ID: covidwho-1931494
ABSTRACT
The majority of metabolomics studies to date have utilised blood serum or plasma, biofluids that do not necessarily address the full range of patient pathologies. Here, correlations between serum metabolites, salivary metabolites and sebum lipids are studied for the first time. 83 COVID-19 positive and negative hospitalised participants provided blood serum alongside saliva and sebum samples for analysis by liquid chromatography mass spectrometry. Widespread alterations to serum-sebum lipid relationships were observed in COVID-19 positive participants versus negative controls. There was also a marked correlation between sebum lipids and the immunostimulatory hormone dehydroepiandrosterone sulphate in the COVID-19 positive cohort. The biofluids analysed herein were also compared in terms of their ability to differentiate COVID-19 positive participants from controls; serum performed best by multivariate analysis (sensitivity and specificity of 0.97), with the dominant changes in triglyceride and bile acid levels, concordant with other studies identifying dyslipidemia as a hallmark of COVID-19 infection. Sebum performed well (sensitivity 0.92; specificity 0.84), with saliva performing worst (sensitivity 0.78; specificity 0.83). These findings show that alterations to skin lipid profiles coincide with dyslipidaemia in serum. The work also signposts the potential for integrated biofluid analyses to provide insight into the whole-body atlas of pathophysiological conditions.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Sebum / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-16123-4

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Sebum / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-16123-4