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Epigallocatechin gallate (EGCG) attenuates severe acute respiratory coronavirus disease 2 (SARS-CoV-2) infection by blocking the interaction of SARS-CoV-2 spike protein receptor-binding domain to human angiotensin-converting enzyme 2.
Ohishi, Tomokazu; Hishiki, Takayuki; Baig, Mirza S; Rajpoot, Sajjan; Saqib, Uzma; Takasaki, Tomohiko; Hara, Yukihiko.
  • Ohishi T; Institute of Microbial Chemistry (BIKAKEN), Numazu, Microbial Chemistry Research Foundation, Numazu-shi, Shizuoka, Japan.
  • Hishiki T; Kanagawa Prefectural Institute of Public Health, Chigasaki, Kanagawa, Japan.
  • Baig MS; Department of Biosciences and Biomedical Engineering (BSBE), Indian Institute of Technology (IIT), Simrol, Indore, India.
  • Rajpoot S; Department of Biosciences and Biomedical Engineering (BSBE), Indian Institute of Technology (IIT), Simrol, Indore, India.
  • Saqib U; Department of Chemistry, Indian Institute of Technology (IIT), Simrol, Indore, India.
  • Takasaki T; Kanagawa Prefectural Institute of Public Health, Chigasaki, Kanagawa, Japan.
  • Hara Y; Tea Solutions, Hara Office Inc., Sumida-ku, Tokyo, Japan.
PLoS One ; 17(7): e0271112, 2022.
Article in English | MEDLINE | ID: covidwho-1933379
ABSTRACT
The outbreak of the coronavirus disease 2019 caused by the severe acute respiratory syndrome coronavirus 2 triggered a global pandemic where control is needed through therapeutic and preventive interventions. This study aims to identify natural compounds that could affect the fusion between the viral membrane (receptor-binding domain of the severe acute respiratory syndrome coronavirus 2 spike protein) and the human cell receptor angiotensin-converting enzyme 2. Accordingly, we performed the enzyme-linked immunosorbent assay-based screening of 10 phytochemicals that already showed numerous positive effects on human health in several epidemiological studies and clinical trials. Among these phytochemicals, epigallocatechin gallate, a polyphenol and a major component of green tea, could effectively inhibit the interaction between the receptor-binding domain of the severe acute respiratory syndrome coronavirus 2 spike protein and the human cell receptor angiotensin-converting enzyme 2. Alternately, in silico molecular docking studies of epigallocatechin gallate and angiotensin-converting enzyme 2 indicated a binding score of -7.8 kcal/mol and identified a hydrogen bond between R393 and angiotensin-converting enzyme 2, which is considered as a key interacting residue involved in binding with the severe acute respiratory syndrome coronavirus 2 spike protein receptor-binding domain, suggesting the possible blocking of interaction between receptor-binding domain and angiotensin-converting enzyme 2. Furthermore, epigallocatechin gallate could attenuate severe acute respiratory syndrome coronavirus 2 infection and replication in Caco-2 cells. These results shed insight into identification and validation of severe acute respiratory syndrome coronavirus 2 entry inhibitors.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Catechin / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / SARS-CoV-2 / COVID-19 / COVID-19 Drug Treatment Type of study: Observational study / Prognostic study Limits: Humans Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2022 Document Type: Article Affiliation country: Journal.pone.0271112

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Catechin / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / SARS-CoV-2 / COVID-19 / COVID-19 Drug Treatment Type of study: Observational study / Prognostic study Limits: Humans Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2022 Document Type: Article Affiliation country: Journal.pone.0271112