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Structural diversity of the SARS-CoV-2 Omicron spike.
Gobeil, Sophie M-C; Henderson, Rory; Stalls, Victoria; Janowska, Katarzyna; Huang, Xiao; May, Aaron; Speakman, Micah; Beaudoin, Esther; Manne, Kartik; Li, Dapeng; Parks, Rob; Barr, Maggie; Deyton, Margaret; Martin, Mitchell; Mansouri, Katayoun; Edwards, Robert J; Eaton, Amanda; Montefiori, David C; Sempowski, Gregory D; Saunders, Kevin O; Wiehe, Kevin; Williams, Wilton; Korber, Bette; Haynes, Barton F; Acharya, Priyamvada.
  • Gobeil SM; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Henderson R; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Medicine, Duke University, Durham, NC 27710, USA.
  • Stalls V; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Janowska K; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Huang X; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • May A; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Biochemistry, Duke University, Durham, NC 27710, USA.
  • Speakman M; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Beaudoin E; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Manne K; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Li D; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Parks R; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Barr M; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Deyton M; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Martin M; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Mansouri K; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Edwards RJ; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Medicine, Duke University, Durham, NC 27710, USA.
  • Eaton A; Duke Human Vaccine Institute, Durham, NC 27710, USA.
  • Montefiori DC; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA.
  • Sempowski GD; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Medicine, Duke University, Durham, NC 27710, USA.
  • Saunders KO; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA.
  • Wiehe K; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Medicine, Duke University, Durham, NC 27710, USA.
  • Williams W; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA.
  • Korber B; Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
  • Haynes BF; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Medicine, Duke University, Durham, NC 27710, USA; Department of Immunology, Duke University, Durham, NC 27710, USA. Electronic address: barton.haynes@duke.edu.
  • Acharya P; Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Biochemistry, Duke University, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA. Electronic address: priyamvada.acharya@duke.edu.
Mol Cell ; 82(11): 2050-2068.e6, 2022 06 02.
Article in English | MEDLINE | ID: covidwho-1937002
ABSTRACT
Aided by extensive spike protein mutation, the SARS-CoV-2 Omicron variant overtook the previously dominant Delta variant. Spike conformation plays an essential role in SARS-CoV-2 evolution via changes in receptor-binding domain (RBD) and neutralizing antibody epitope presentation, affecting virus transmissibility and immune evasion. Here, we determine cryo-EM structures of the Omicron and Delta spikes to understand the conformational impacts of mutations in each. The Omicron spike structure revealed an unusually tightly packed RBD organization with long range impacts that were not observed in the Delta spike. Binding and crystallography revealed increased flexibility at the functionally critical fusion peptide site in the Omicron spike. These results reveal a highly evolved Omicron spike architecture with possible impacts on its high levels of immune evasion and transmissibility.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Variants Limits: Humans Language: English Journal: Mol Cell Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: J.molcel.2022.03.028

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Variants Limits: Humans Language: English Journal: Mol Cell Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: J.molcel.2022.03.028