Differences in endosomal Rab gene expression between positive and negative COVID-19 patients.
BMC Res Notes
; 15(1): 252, 2022 Jul 15.
Article
in English
| MEDLINE | ID: covidwho-1938343
ABSTRACT
OBJECTIVE:
SARS CoV-2, the etiologic agent of coronavirus disease-2019 (COVID-19) is well-known to use ACE2 to begin internalization. Some viruses enter the host cell through the endocytosis process and involve some endocytosis proteins, such as the Rab family. However, the relationship between SARS CoV-2 infection with endocytic mRNA RAB5, RAB7, and RAB11B is unknown. This study aims to compare the expression of RAB5, RAB7, and RAB11B between positive and negative COVID-19 patient groups.RESULTS:
Both viral and human epithelial RNA Isolation and RT-PCR were performed from 249 samples. The genes expression was analysed using appropriate statistical tests. We found the Median (inter-quartile range/IQR) of RAB5, RAB7, and RAB11B expression among the COVID-19 patient group was 2.99 (1.88), 0.17 (0.47), 0.47 (1.49), and 1.60 (2.88), 1.05 (2.49), 1.10 (3.96) among control group respectively. We proceeded with Mann Whitney U Test and found that RAB5 expression was significantly increased (P < 0.001), and RAB7 and RAB11B expression was significantly decreased (P < 0.001 and P = 0.036) in the COVID-19 patient group compared to the control group. This first report showed significant differences in RAB5, RAB7, and RAB11B exist between COVID-19 positive and negative patients.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Rab5 GTP-Binding Proteins
/
COVID-19
Type of study:
Etiology study
/
Experimental Studies
/
Randomized controlled trials
Limits:
Humans
Language:
English
Journal:
BMC Res Notes
Year:
2022
Document Type:
Article
Affiliation country:
S13104-022-06144-7
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