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Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases.
Guest, Paul C; Abbasifard, Mitra; Jamialahmadi, Tannaz; Majeed, Muhammed; Kesharwani, Prashant; Sahebkar, Amirhossein.
  • Guest PC; Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
  • Abbasifard M; Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. abbasifardm@yandex.com.
  • Jamialahmadi T; Department of Internal Medicine, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. abbasifardm@yandex.com.
  • Majeed M; Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Kesharwani P; Sabinsa Corporation, East Windsor, NJ, USA.
  • Sahebkar A; Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
Methods Mol Biol ; 2511: 245-256, 2022.
Article in English | MEDLINE | ID: covidwho-1941380
ABSTRACT
Severe cases of SARS-CoV-2 and other pathogenic virus infections are often associated with the uncontrolled release of proinflammatory cytokines, known as a "cytokine storm." We present a protocol for multiplex analysis of three cytokines, tumor necrosis factor-alpha (TNF-a), interleukin 6 (IL-6), and IL-10, which are typically elevated in cytokine storm events and may be used as a predictive biomarker profile of disease severity or disease course.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cytokine Release Syndrome / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: 978-1-0716-2395-4_18

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cytokine Release Syndrome / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: 978-1-0716-2395-4_18