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Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs).
Iwata, Keiko; Xie, Min-Jue; Guest, Paul C; Hirai, Takaharu; Matsuzazki, Hideo.
  • Iwata K; Division of Development of Mental Functions, Research Center for Child Mental Development, University of Fukui, Fukui, Japan. kiwata@u-fukui.ac.jp.
  • Xie MJ; United Graduate School of Child Development, Osaka University, Osaka, Japan. kiwata@u-fukui.ac.jp.
  • Guest PC; Life Science Innovation Center, University of Fukui, Fukui, Japan. kiwata@u-fukui.ac.jp.
  • Hirai T; Division of Development of Mental Functions, Research Center for Child Mental Development, University of Fukui, Fukui, Japan.
  • Matsuzazki H; United Graduate School of Child Development, Osaka University, Osaka, Japan.
Methods Mol Biol ; 2511: 321-332, 2022.
Article in English | MEDLINE | ID: covidwho-1941386
ABSTRACT
Inflammatory diseases caused by infectious agents such as the SARS-CoV-2 virus can lead to impaired reductive-oxidative (REDOX) balance and disrupted mitochondrial function. Peripheral blood mononuclear cells (PBMCs) provide a useful model for studying the effects of inflammatory diseases on mitochondrial function but can be limited by the need to store these cells by cryopreservation prior to assay. Here, we describe a method for improving and determining PBMC viability with normalization of values to number of living cells. The approach can be applied not only to PBMC samples derived from patients with diseases marked by an altered inflammatory response such as viral infections.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Leukocytes, Mononuclear / COVID-19 Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: 978-1-0716-2395-4_24

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Leukocytes, Mononuclear / COVID-19 Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: 978-1-0716-2395-4_24