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Optimized intramuscular immunization with VSV-vectored spike protein triggers a superior immune response to SARS-CoV-2.
Taddeo, Adriano; Veiga, Inês Berenguer; Devisme, Christelle; Boss, Renate; Plattet, Philippe; Weigang, Sebastian; Kochs, Georg; Thiel, Volker; Benarafa, Charaf; Zimmer, Gert.
  • Taddeo A; Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland.
  • Veiga IB; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Devisme C; Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland.
  • Boss R; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Plattet P; Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland.
  • Weigang S; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Kochs G; Federal Food Safety and Veterinary Office, Bern, Switzerland.
  • Thiel V; Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Benarafa C; Institute of Virology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • Zimmer G; Institute of Virology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
NPJ Vaccines ; 7(1): 82, 2022 Jul 25.
Article in English | MEDLINE | ID: covidwho-1956404
ABSTRACT
Immunization with vesicular stomatitis virus (VSV)-vectored COVID-19 vaccine candidates expressing the SARS-CoV-2 spike protein in place of the VSV glycoprotein relies implicitly on expression of the ACE2 receptor at the muscular injection site. Here, we report that such a viral vector vaccine did not induce protective immunity following intramuscular immunization of K18-hACE2 transgenic mice. However, when the viral vector was trans-complemented with the VSV glycoprotein, intramuscular immunization resulted in high titers of spike-specific neutralizing antibodies. The vaccinated animals were fully protected following infection with a lethal dose of SARS-CoV-2-SD614G via the nasal route, and partially protected if challenged with the SARS-CoV-2Delta variant. While dissemination of the challenge virus to the brain was completely inhibited, replication in the lung with consequent lung pathology was not entirely controlled. Thus, intramuscular immunization was clearly enhanced by trans-complementation of the VSV-vectored vaccines by the VSV glycoprotein and led to protection from COVID-19, although not achieving sterilizing immunity.

Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Topics: Vaccines / Variants Language: English Journal: NPJ Vaccines Year: 2022 Document Type: Article Affiliation country: S41541-022-00508-7

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Topics: Vaccines / Variants Language: English Journal: NPJ Vaccines Year: 2022 Document Type: Article Affiliation country: S41541-022-00508-7