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Severe COVID-19 correlates with lower mitochondrial cristae density in PBMCs and greater sitting time in humans.
Castro-Sepulveda, Mauricio; Tapia, German; Tuñón-Suárez, Mauro; Diaz, Alejandra; Marambio, Hugo; Valero-Breton, Mayalen; Fernández-Verdejo, Rodrigo; Zbinden-Foncea, Hermann.
  • Castro-Sepulveda M; Exercise Physiology and Metabolism Laboratory (LABFEM), School of Kinesiology, Faculty of Medicine, Finis Terrae University, Santiago, Chile.
  • Tapia G; Exercise Physiology and Metabolism Laboratory (LABFEM), School of Kinesiology, Faculty of Medicine, Finis Terrae University, Santiago, Chile.
  • Tuñón-Suárez M; Sports Health Center, Santa María Clinic, Santiago, Chile.
  • Diaz A; Exercise Physiology and Metabolism Laboratory (LABFEM), School of Kinesiology, Faculty of Medicine, Finis Terrae University, Santiago, Chile.
  • Marambio H; Sports Health Center, Santa María Clinic, Santiago, Chile.
  • Valero-Breton M; Sports Health Center, Santa María Clinic, Santiago, Chile.
  • Fernández-Verdejo R; Exercise Physiology and Metabolism Laboratory (LABFEM), School of Kinesiology, Faculty of Medicine, Finis Terrae University, Santiago, Chile.
  • Zbinden-Foncea H; Exercise Physiology and Metabolism Laboratory (LABFEM), School of Kinesiology, Faculty of Medicine, Finis Terrae University, Santiago, Chile.
Physiol Rep ; 10(14): e15369, 2022 07.
Article in English | MEDLINE | ID: covidwho-1957609
ABSTRACT
An interaction between mitochondrial dynamics, physical activity levels, and COVID-19 severity has been previously hypothesized. However, this has not been tested. We aimed to compare mitochondrial morphology and cristae density of PBMCs between subjects with non-severe COVID-19, subjects with severe COVID-19, and healthy controls. Additionally, we compared the level of moderate-vigorous physical activity (MVPA) and sitting time between groups. Blood samples were taken to obtain PBMCs. Mitochondrial dynamics were assessed by electron microscopy images and western blot of protein that regulate mitochondrial dynamics. The International Physical Activity Questionnaire (IPAQ; short version) was used to estimate the level of MVPA and the sitting time The patients who develop severe COVID-19 (COVID-19++) not present alterations of mitochondrial size neither mitochondrial density in comparison to non-severe patients COVID-19 (COVID-19) and control subjects (CTRL). However, compared to CTRL, COVID-19 and COVID-19++ groups have lower mitochondrial cristae length, a higher proportion of abnormal mitochondrial cristae. The COVID-19++ group has lower number (trend) and length of mitochondrial cristae in comparison to COVID-19 group. COVID-19, but not COVID-19++ group had lower Opa 1, Mfn 2 and SDHB (Complex II) proteins than CTRL group. Besides, COVID-19++ group has a higher time sitting. Our results show that low mitochondrial cristae density, potentially due to physical inactivity, is associated with COVID-19 severity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Sitting Position / COVID-19 Type of study: Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Physiol Rep Year: 2022 Document Type: Article Affiliation country: Phy2.15369

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Sitting Position / COVID-19 Type of study: Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Physiol Rep Year: 2022 Document Type: Article Affiliation country: Phy2.15369